ArticleApplied and environmental microbiology2012
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Effects of levels of purity of spores of twoApplied and environmental microbiology · 2026Article
- Sporicidal and Anti-germinative Effects of Achyrocline satureioides Extract on Paenibacillus larvae: A Potential Natural Strategy for American Foulbrood Control.Current microbiology · 2026Article
- Engineered Lactate Catabolizing Probiotics Reveal Timescale Dependent Microbiome-Host Metabolic Coupling.bioRxiv : the preprint server for biology · 2026Article
- Effects of sporulation times in liquid or on plates onJournal of bacteriology · 2025Article
- Sporulation at reduced water activity impairs germination kinetics ofApplied and environmental microbiology · 2025Article
- Germination and Heat Resistance ofFoods (Basel, Switzerland) · 2025Article
- Amino Acids From Root Exudates Induce Bacillus Spore Germination to Enhance Root Colonisation and Plant Growth Promotion.Microbial biotechnology · 2025Article
- Phosphorelay changes and plasticity underlie the life history evolution ofMicrobiology (Reading, England) · 2025Article
- EngineeredACS synthetic biology · 2025Article
- GerAB residues predicted to interfere with water passage based on steered molecular dynamics are key to germinosome functionality.Frontiers in microbiology · 2025Article
- Identification and characterization of theJournal of bacteriology · 2024Article
- Influence of Sporulation Temperature on Germination and Growth ofFoods (Basel, Switzerland) · 2024Article
- L-Alanine promotes anti-infectious properties of Bacillus subtilis S-2 spores via the germination receptor gerAA.Probiotics and antimicrobial proteins · 2024Article
- EngineeredbioRxiv : the preprint server for biology · 2024Article
- A Family of Spore Lipoproteins Stabilizes the Germination Apparatus by Altering Inner Spore Membrane Fluidity inJournal of bacteriology · 2023Article
- Bacterial spore germination receptors are nutrient-gated ion channels.Science (New York, N.Y.) · 2023Article
- Comparison of sporulation and germination conditions forFrontiers in microbiology · 2023Article
- Glutamate catabolism during sporulation determines the success of the future spore germination.iScience · 2022Article
- Effect of oxygen on the germination and culturability of Bacillus atrophaeus spores.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Mechanisms and Applications of Bacterial Sporulation and Germination in the Intestine.International journal of molecular sciences · 2022Review
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis spores prepared in rich medium germinated faster with nutrient germinants than poor-medium spores as populations in liquid and multiple individual spores on a microscope slide. Poor-medium spores had longer average lag times between mixing of spores with nutrient germinants and initiation of Ca-dipicolinic acid (CaDPA) release. Rich-medium spores made at 37°C germinated slightly faster with nutrient germinants than 23°C spores in liquid, but not when spores germinated on a slide. The difference in germination characteristics of these spore populations in liquid was paralleled by changes in expression levels of a transcriptional lacZ fusion to the gerA operon, encoding a germinant receptor (GR). Levels of GR subunits were 3- to 8-fold lower in poor-medium spores than rich-medium spores and 1.6- to 2-fold lower in 23°C spores than 37°C spores, and levels of the auxiliary germination protein GerD were 3.5- to 4-fold lower in poor medium and 23°C spores. In contrast, levels of another likely germination protein, SpoVAD, were similar in all these spores. These different spores germinated similarly with CaDPA, and poor-medium and 23°C spores germinated faster than rich-medium and 37°C spores, respectively, with dodecylamine. Since spore germination with CaDPA and dodecylamine does not require GerD or GRs, these results indicate that determinants of rates of nutrient germination of spores prepared differently are primarily the levels of the GRs that bind nutrient germinants and trigger germination and secondarily the levels of GerD.
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