ArticleApplied and environmental microbiology2012
Rapid and specific detection of escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Article
- Article
- Rapid and visual detection of Shiga-toxigenic Escherichia coli (STEC) in carabeef meat harnessing loop-mediated isothermal amplification (LAMP).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Clinical, Molecular and Serological Diagnosis of Canine Leishmaniosis: An Integrated Approach.Veterinary sciences · 2020Article
- Viable but Nonculturable Escherichia coli O157:H7 and Salmonella enterica in Fresh Produce: Rapid Determination by Loop-Mediated Isothermal Amplification Coupled with a Propidium Monoazide Treatment.Applied and environmental microbiology · 2020Article
- Loop-mediated isothermal amplification: rapid molecular detection of virulence genes associated with avian pathogenic Escherichia coli in poultry.Poultry science · 2019Article
- Three Dimensional Vero Cell-Platform for Rapid and Sensitive Screening of Shiga-Toxin ProducingFrontiers in microbiology · 2019Article
- Evaluation and improvement of LAMP assays for detection ofAfrican health sciences · 2017Article
- Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time.BMC microbiology · 2016Article
- Sensitive detection of Escherichia coli O157:H7 using Pt-Au bimetal nanoparticles with peroxidase-like amplification.Biosensors & bioelectronics · 2016Article
- Nucleic acid-based biotechnologies for food-borne pathogen detection using routine time-intensive culture-based methods and fast molecular diagnostics.Food science and biotechnology · 2016Review
- Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus.Iranian journal of microbiology · 2015Article
- Quantitative determination of target gene with electrical sensor.Scientific reports · 2015Article
- A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria.Molecules (Basel, Switzerland) · 2015Article
- Comparison of whole genome sequences from human and non-human Escherichia coli O26 strains.Frontiers in cellular and infection microbiology · 2015Article
- Electrical detection of nucleic acid amplification using an on-chip quasi-reference electrode and a PVC REFET.Analytical chemistry · 2014Article
- Evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of Shiga toxin-producing Escherichia coli in produce.Applied and environmental microbiology · 2014Article
- Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle.Journal of veterinary science · 2014Article
- Detection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS).Food microbiology · 2013Article
- Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification.ISRN bioinformatics · 2012Article
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Authors and funding
5 authors.
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Abstract
Escherichia coli O157 and six additional serogroups of Shiga toxin-producing E. coli (STEC) (O26, O45, O103, O111, O121, and O145) account for the majority of STEC infections in the United States. In this study, O serogroup-specific genes (wzx or wzy) were used to design loop-mediated isothermal amplification (LAMP) assays for the rapid and specific detection of these leading STEC serogroups. The assays were evaluated in pure culture and spiked food samples (ground beef, beef trim, lettuce, and spinach) and compared with real-time quantitative PCR (qPCR). No false-positive or false-negative results were observed among 120 bacterial strains used to evaluate assay specificity. The limits of detection of various STEC strains belonging to these target serogroups were approximately 1 to 20 CFU/reaction mixture in pure culture and 10(3) to 10(4) CFU/g in spiked food samples, which were comparable to those of qPCR. Standard curves generated suggested good linear relationships between STEC cell numbers and LAMP turbidity signals. In various beef and produce samples spiked with two low levels (1 to 2 and 10 to 20 CFU/25 g) of respective STEC strains, the LAMP assays consistently achieved accurate detection after 6 to 8 h of enrichment. In conclusion, these newly developed LAMP assays may facilitate rapid and reliable detection of the seven major STEC serogroups in ground beef, beef trim, and produce during routine sample testing.
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