ArticleApplied and environmental microbiology2012
Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
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- Trace copper-mediated asexual developmentFrontiers in microbiology · 2023Article
- Biological Characteristics ofInternational journal of molecular sciences · 2021Article
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- The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool.Bioresources and bioprocessing · 2021Review
- The master regulator MAT1-1-1 of fungal mating binds to its targets via a conserved motif in the human pathogen Aspergillus fumigatus.G3 (Bethesda, Md.) · 2021Article
- The fungal sexual revolution continues: discovery of sexual development in members of the genus Aspergillus and its consequences.Fungal biology and biotechnology · 2020Article
- Classification ofStudies in mycology · 2020Article
- Genome-Wide Analyses of Repeat-Induced Point Mutations in the Ascomycota.Frontiers in microbiology · 2020Article
- Evolution of Aspergillus oryzae before and after domestication inferred by large-scale comparative genomic analysis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Identification of new signalling peptides through a genome-wide survey of 250 fungal secretomes.BMC genomics · 2019Article
- Evolution of asexual and sexual reproduction in the aspergilli.Studies in mycology · 2018Article
- BiFC-based visualisation system reveals cell fusion morphology and heterokaryon incompatibility in the filamentous fungus Aspergillus oryzae.Scientific reports · 2018Article
- Volatiles Mediated Interactions BetweenFrontiers in microbiology · 2018Article
- Mating-type genes of the anamorphic fungus Ulocladium botrytis affect both asexual sporulation and sexual reproduction.Scientific reports · 2017Article
- Sex and the Imperfect Fungi.Microbiology spectrum · 2017Review
- Characterization of Non-coding Regions in B Mating Loci of Agrocybe salicacola Groups: Target Sites for B Mating Type Identification.Current microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the reference strain RIB40. We now report the existence of a complementary MAT1-2 gene and the sequencing of an idiomorphic region from A. oryzae strain AO6. This allowed the development of a PCR diagnostic assay, which detected isolates of the MAT1-1 and MAT1-2 genotypes among 180 strains assayed, including industrial tane-koji isolates. Strains used for sake and miso production showed a near-1:1 ratio of the MAT1-1 and MAT1-2 mating types, whereas strains used for soy sauce production showed a significant bias toward the MAT1-2 mating type. MAT1-1 and MAT1-2 isogenic strains were then created by genetic manipulation of the resident idiomorph, and gene expression was compared by DNA microarray and quantitative real-time PCR (qRT-PCR) methodologies under conditions in which MAT genes were expressed. Thirty-three genes were found to be upregulated more than 10-fold in either the MAT1-1 host strain or the MAT1-2 gene replacement strain relative to each other, showing that both the MAT1-1 and MAT1-2 genes functionally regulate gene expression in A. oryzae in a mating type-dependent manner, the first such report for a supposedly asexual fungus. MAT1-1 expression specifically upregulated an α-pheromone precursor gene, but the functions of most of the genes affected were unknown. The results are consistent with a heterothallic breeding system in A. oryzae, and prospects for the discovery of a sexual cycle are discussed.
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