ArticleIntegrative biology : quantitative biosciences from nano to macro2012
Calibrating spatio-temporal models of leukocyte dynamics against in vivo live-imaging data using approximate Bayesian computation.
Article in Integrative biology : quantitative biosciences from nano to macro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Drosophila complement-like Mcr acts as a wound-induced inflammatory chemoattractant.Current biology : CB · 2025Article
- Spatial considerations in the resolution of inflammation: Elucidating leukocyte interactions via an experimentally-calibrated agent-based model.PLoS computational biology · 2020Article
- The cell biology of inflammation: From common traits to remarkable immunological adaptations.The Journal of cell biology · 2020Review
- Mechanisms and Points of Control in the Spread of Inflammation: A Mathematical Investigation.Bulletin of mathematical biology · 2020Article
- The impact of temporal sampling resolution on parameter inference for biological transport models.PLoS computational biology · 2018Article
- Nonmotile Single-Cell Migration as a Random Walk in Nonuniformity: The "Extreme Dumping Limit" for Cell-to-Cell Communications.Journal of healthcare engineering · 2018Article
- Systems Analysis of the Dynamic Inflammatory Response to Tissue Damage Reveals Spatiotemporal Properties of the Wound Attractant Gradient.Current biology : CB · 2016Article
- Article
- Optimal experiment design for model selection in biochemical networks.BMC systems biology · 2014Article
- A framework for parameter estimation and model selection from experimental data in systems biology using approximate Bayesian computation.Nature protocols · 2014Article
- Approximate Bayesian inference for complex ecosystems.F1000prime reports · 2014Review
- PhagoSight: an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model.PloS one · 2013Article
- Maximizing the information content of experiments in systems biology.PLoS computational biology · 2013Article
- P38 and JNK have opposing effects on persistence of in vivo leukocyte migration in zebrafish.Immunology and cell biology · 2013Article
- Repelled from the wound, or randomly dispersed? Reverse migration behaviour of neutrophils characterized by dynamic modelling.Journal of the Royal Society, Interface · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
In vivo studies allow us to investigate biological processes at the level of the organism. But not all aspects of in vivo systems are amenable to direct experimental measurements. In order to make the most of such data we therefore require statistical tools that allow us to obtain reliable estimates for e.g. kinetic in vivo parameters. Here we show how we can use approximate Bayesian computation approaches in order to analyse leukocyte migration in zebrafish embryos in response to injuries. We track individual leukocytes using live imaging following surgical injury to the embryos' tail-fins. The signalling gradient that leukocytes follow towards the site of the injury cannot be directly measured but we can estimate its shape and how it changes with time from the directly observed patterns of leukocyte migration. By coupling simple models of immune signalling and leukocyte migration with the unknown gradient shape into a single statistical framework we can gain detailed insights into the tissue-wide processes that are involved in the innate immune response to wound injury. In particular we find conclusive evidence for a temporally and spatially changing signalling gradient that modulates the changing activity of the leukocyte population in the embryos. We conclude with a robustness analysis which highlights the most important factors determining the leukocyte dynamics. Our approach relies only on the ability to simulate numerically the process under investigation and is therefore also applicable in other in vivo contexts and studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.