ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2012
HSP90 inhibition by 17-DMAG reduces inflammation in J774 macrophages through suppression of Akt and nuclear factor-κB pathways.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Pharmacological effects of the Cassia Seed on atherosclerosis: A meta-analysis based on network pharmacology.Medicine · 2022Pooled it
- Natural small molecule smart hydrogels inhibited the Hsp90/NF-κB signaling axis in inflammation to achieve sustained antipyretic effect.Journal of nanobiotechnology · 2025Article
- Comprehensive analysis of heat shock proteins in glioma revealed the association with glioma-associated myeloid cells.Genes and immunity · 2025Article
- HSP90 promotes tumor associated macrophage differentiation during triple-negative breast cancer progression.Scientific reports · 2024Article
- Intraperitoneal Administration of 17-DMAG as an Effective Treatment againstPathogens (Basel, Switzerland) · 2024Article
- Time-Course Transcriptome Analysis of the Lungs of Mice Challenged with Aerosols of Methicillin-ResistantBiomolecules · 2023Article
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- Heat shock proteins and viral infection.Frontiers in immunology · 2022Review
- Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study.Drug design, development and therapy · 2022Article
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- The HSP Immune Network in Cancer.Frontiers in immunology · 2021Review
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- Annexin A2 depletion exacerbates the intracerebral microhemorrhage induced by acute rickettsia and Ebola virus infections.PLoS neglected tropical diseases · 2020Article
- Article
- Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages.Journal of leukocyte biology · 2017Article
- Apoptosis in response to heat stress is positively associated with heat-shock protein 90 expression in chicken myocardial cellsJournal of veterinary science · 2017Article
- Identification of key genes and pathways for peri-implantitis through the analysis of gene expression data.Experimental and therapeutic medicine · 2017Article
- Anti-Inflammatory Mechanism Involved in Pomegranate-Mediated Prevention of Breast Cancer: the Role of NF-κB and Nrf2 Signaling Pathways.Nutrients · 2017Article
- Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.Oncotarget · 2016Article
- FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in mice.Nature communications · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveThis study was designed to determine whether inhibition of heat shock protein 90 (HSP90) reduces pro-inflammatory mediator production by decreasing the nuclear factor (NF)-κB and Akt signaling pathways in immune-stimulated macrophages.
methodsJ774A.1 murine macrophages were treated with the HSP90 inhibitor 17-DMAG (0.01, 0.1 or 1 μM) prior to immune stimulation with lipopolysaccharide and interferon-γ. Expression of Akt, inhibitor of κB kinase (IKK), and heat shock proteins were measured in whole cell lysates by Western blotting. Phosphorylated Akt and inhibitor of κB (IκB) were measured in whole cell lysates by ELISA. Cell supernatants were analyzed for interleukin (IL)-6, tumor necrosis factor (TNF)-α and nitric oxide (NO). Translocation of NF-κB and heat shock factor (HSF)-1 was assessed by immunofluorescence.
resultsTreating cells with 17-DMAG reduced expression of Akt and IKK in immune-stimulated cells. 17-DMAG reduced nuclear translocation of NF-κB and reduced immune-stimulated production of IL-6, TNF-α and NO, but did not decrease inducible nitric oxide synthase expression.
conclusionsOur studies show that the immune-mediated NF-κB inflammatory cascade is blocked by the HSP90 inhibitor 17-DMAG. Due to the broad interaction of HSP90 with many pro-inflammatory kinase cascades, inhibition of HSP90 may provide a novel approach to reducing chronic inflammation.
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