ArticleVeterinary research communications2012
Could fetal fluid and membranes be an alternative source for mesenchymal stem cells (MSCs) in the feline species? A preliminary study.
Article in Veterinary research communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- Mesenchymal Stem Cells: Generalities and Clinical Significance in Feline and Canine Medicine.Animals : an open access journal from MDPI · 2023Review
- Feline umbilical cord-derived mesenchymal stem cells: isolation, identification, and antioxidative stress role through NF-κB signaling pathway.Frontiers in veterinary science · 2023Article
- Isolation and Characterization of Feline Wharton's Jelly-Derived Mesenchymal Stem Cells.Veterinary sciences · 2021Article
- Proteomic Analysis of the Secretome and Exosomes of Feline Adipose-Derived Mesenchymal Stem Cells.Animals : an open access journal from MDPI · 2021Article
- Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp.Scientific reports · 2020Article
- Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.Bioscience reports · 2020Article
- Mesenchymal stem cell therapy in cats: Current knowledge and future potential.Journal of feline medicine and surgery · 2018Review
- Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells.Experimental animals · 2016Article
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- Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation.Stem cells and cloning : advances and applications · 2014Article
- Molecular characterization and in vitro differentiation of feline progenitor-like amniotic epithelial cells.Stem cell research & therapy · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Domestic cats are preferred models for normal physiology and several human diseases. In the present study feline fetal fluids and membranes were evaluated as possible sources of MSCs. Samples were recovered from 4 pregnant queens after ovarian-hysterectomy. Gestational sacs were separated from uterine wall; after allantoic and amniotic fluids aspiration and chorion-allantois and amniotic membranes separation, all cell lineages were cultured into 25 cm(2) flasks, in DMEM/TCM199, in a 5% CO(2) incubator at 38.5 °C. At passage 3, chondrogenic, osteogenic and adipogenic differentiation ability were evaluated by culturing cell monolayers in differentiating media for 21 days. Cellular characterization with CD90, CD44, CD105, CD73, CD34, CD14, CD45, was performed by flow cytometry. In all samples, adherent fibroblastoid spindle-shaped cells were observed. Positive von Kossa and Alizarin Red staining confirmed osteogenesis. Alcian blue staining of matrix glycosaminoglycans illustrated chondrogenesis, and positive Oil Red O lipid droplets within cell cytoplasm suggested adipogenesis. All cell lines isolated were positive for CD90, CD44, CD105 and negative for CD34, CD14 and CD45; as unexpected and different from human cells, feline cells resulted negative for CD73. Based on this preliminary results, fetal fluids and membranes could represent an alternative sources for mesenchymal stem cells in feline species.
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Registered trials
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