ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2012
Evaluation of a digital microfluidic real-time PCR platform to detect DNA of Candida albicans in blood.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 3 syntheses or guidelines pooled it, 80 citations in OpenAlex.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Digital microfluidics comes of age: high-throughput screening to bedside diagnostic testing for genetic disorders in newborns.Expert review of molecular diagnostics · 2018Pooled it
- Clinical Practice Guideline for the Management of Candidiasis: 2016 Update by the Infectious Diseases Society of America.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Guideline
- Nucleic acid amplification tests in digital microfluidics: the promise of next-generation point-of-care diagnostics.Microsystems & nanoengineering · 2025Review
- Unified metagenomic method for rapid detection of microorganisms in clinical samples.Communications medicine · 2024Article
- Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip.ACS omega · 2024Article
- Innovative Biosensing Approaches for Swift Identification ofLife (Basel, Switzerland) · 2023Review
- Performance of a Real-Time PCR Assay for the Detection of FiveJournal of fungi (Basel, Switzerland) · 2023Article
- Performance of Multiplex PCR and β-1,3-D-Glucan Testing for the Diagnosis of Candidemia.Journal of fungi (Basel, Switzerland) · 2022Article
- Clinical validation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections in ICU practice: a promising diagnostic tool.Critical care (London, England) · 2022Article
- Application of Micro/Nanoporous Fluoropolymers with Reduced Bioadhesion in Digital Microfluidics.Nanomaterials (Basel, Switzerland) · 2022Article
- Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Recent Advances and Novel Approaches in Laboratory-Based Diagnostic Mycology.Journal of fungi (Basel, Switzerland) · 2021Review
- Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples.Frontiers in microbiology · 2021Article
- Molecular Diagnosis of Yeast Infections.Current fungal infection reports · 2021Review
- A Routing-Based Repair Method for Digital Microfluidic Biochips Based on an Improved Dijkstra and Improved Particle Swarm Optimization Algorithm.Micromachines · 2020Article
- A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Microfluidic Chip for Detection of Fungal Infections.ACS omega · 2019Article
- Direct Detection ofSensors (Basel, Switzerland) · 2018Article
- Digital Microfluidics for Nucleic Acid Amplification.Sensors (Basel, Switzerland) · 2017Review
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
Abstract
Species of Candida frequently cause life-threatening infections in neonates, transplant and intensive care unit (ICU) patients, and others with compromised host defenses. The successful management of systemic candidiasis depends upon early, rapid diagnosis. Blood cultures are the standard diagnostic method, but identification requires days and less than half of the patients are positive. These limitations may be eliminated by using real-time polymerase chain reaction (PCR) to detect Candida DNA in the blood specimens of patients at risk. Here, we optimized a PCR protocol to detect 5-10 yeasts in low volumes of simulated and clinical specimens. We also used a mouse model of systemic candidiasis and determined that candidemia is optimally detectable during the first few days after infection. However, PCR tests are often costly, labor-intensive, and inconvenient for routine use. To address these obstacles, we evaluated the innovative microfluidic real-time PCR platform (Advanced Liquid Logic, Inc.), which has the potential for full automation and rapid turnaround. Eleven and nine of 16 specimens from individual patients with culture-proven candidemia tested positive for C. albicans DNA by conventional and microfluidic real-time PCR, respectively, for a combined sensitivity of 94%. The microfluidic platform offers a significant technical advance in the detection of microbial DNA in clinical specimens.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.