ArticleParasitology research2012
A quick and simple method, usable in the field, for collecting parasites in suitable condition for both morphological and molecular studies.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- First record ofInternational journal for parasitology. Parasites and wildlife · 2026Review
- "A Tale Preserved in a Museum": The Long-Awaited Discovery of Genitocotyle necromnemos n. sp. (Trematoda: Opecoelidae) from the Cardinal Fish Apogon imberbis (Linnaeus) in the Western Mediterranean.Acta parasitologica · 2025Article
- Potentially zoonotic parasite arrives to continental France: Clinostomum complanatum (Trematoda: Digenea) infection in perch (Perca fluviatilis) from the Doubs River (Jura).Folia parasitologica · 2025Article
- Helminth absence and invasion success of blackchin tilapia (Frontiers in veterinary science · 2025Article
- Taxonomic inflation or hidden diversity: unveiling the fact surrounds Lytocestus indicus (Cestoda: Caryophyllidea).Parasitology research · 2024Article
- Highly resolved genome assembly and comparative transcriptome profiling reveal genes related to developmental stages of tapewormProceedings. Biological sciences · 2024Article
- Untangling the Derogenes varicus species complex in Scandinavian waters and the Arctic: description of Derogenes abba n. sp. (Trematoda, Derogenidae) from Hippoglossoides platessoides and new host records for D. varicus (Müller, 1784) sensu stricto.Parasite (Paris, France) · 2024Article
- Morphological and molecular studies of the life-cycle stages of the monorchiid Monorchis parvus (Looss, 1902) (Digenea) from the Southern Mediterranean coast (Tunisia).Parasitology research · 2023Article
- Top-down and bottom-up effects and relationships with local environmental factors in the water frog-helminth systems in Latvia.Scientific reports · 2023Article
- First Study onAnimals : an open access journal from MDPI · 2023Article
- Hidden in the fog: morphological and molecular characterisation of Derogenes varicus sensu stricto (Trematoda, Derogenidae) from Sweden and Norway, and redescription of two poorly known Derogenes species.Parasite (Paris, France) · 2023Article
- Temporal stability of polymorphic Arctic charr parasite communities reflects sustained divergent trophic niches.Ecology and evolution · 2022Article
- Physicochemical Water Quality Influence on the Parasite Biodiversity in Juvenile Tilapia (Pathogens (Basel, Switzerland) · 2022Article
- PINWORMS ARE ASSOCIATED WITH TAXONOMIC BUT NOT FUNCTIONAL DIFFERENCES IN THE GUT MICROBIOME OF WHITE-THROATED WOODRATS (NEOTOMA ALBIGULA).The Journal of parasitology · 2022Article
- Description of a new species from Clarias maclareni and phylogenetical analysis of Quadriacanthus (Monogenea, Dactylogyridae) species transfers between clariid and non-clariid fish hosts in Cameroon.Parasite (Paris, France) · 2022Article
- Detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Article
- Development of a Novel Method for Identification ofMicroorganisms · 2021Article
- Conspecific migration and environmental setting determine parasite infracommunities of non-migratory individual fish.Parasitology · 2021Article
- Isotopic discrimination in helminths infecting coral reef fishes depends on parasite group, habitat within host, and host stable isotope value.Scientific reports · 2021Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many methods have been proposed for collecting and fixing parasites, but most were written before the molecular age, and were intended to be practised by experienced parasitologists in well-equipped laboratories. We describe here a very simple method, illustrated by photographs, for collecting helminths from the digestive tract of vertebrates. It only requires a few plastic vials, some ethanol and a means to heat water. Basically, the method consists of: (a) the extraction of all organs from the abdominal cavity; (b) opening the digestive system longitudinally; (c) agitate gut and contents in a saline solution (i.e. ca. 9% NaCl or 1/4 sea water in tap water); (d) decant in saline as many times as needed to clean contents; (e) immediately fix parasites in near-boiling saline; (f) discard saline and keep specimens in 95% ethanol. Additional information is given for collecting parasites from fish gills with a similar process. The method will collect most helminths (digeneans, larval cestodes, nematodes, acanthocephalans) from the digestive tract, and monogeneans and isopod and copepod crustaceans from fish gills. The specimens will be suitable for both morphological study and DNA sequencing. The method is simple, fast, inexpensive and can be used by untrained personnel, even in the field without electricity and without a binocular microscope. It can also be used by trained parasitologists who need to expedite treatment of abundant samples.
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