ArticleEukaryotic cell2012
Wsp1 is downstream of Cin1 and regulates vesicle transport and actin cytoskeleton as an effector of Cdc42 and Rac1 in Cryptococcus neoformans.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Genetic Transformation inJournal of fungi (Basel, Switzerland) · 2021Review
- Transcriptomic Analysis of Extracellular RNA Governed by the Endocytic Adaptor Protein Cin1 ofFrontiers in cellular and infection microbiology · 2020Article
- The TOR Pathway Plays Pleiotropic Roles in Growth and Stress Responses of the Fungal PathogenGenetics · 2019Article
- Ras signalling in pathogenic yeasts.Microbial cell (Graz, Austria) · 2017Review
- Biological properties of extracellular vesicles and their physiological functions.Journal of extracellular vesicles · 2015Article
- Fungal morphogenesis.Cold Spring Harbor perspectives in medicine · 2014Review
- Noncanonical Gβ Gib2 is a scaffolding protein promoting cAMP signaling through functions of Ras1 and Cac1 proteins in Cryptococcus neoformans.The Journal of biological chemistry · 2014Article
- Restricted substrate specificity for the geranylgeranyltransferase-I enzyme in Cryptococcus neoformans: implications for virulence.Eukaryotic cell · 2013Article
- Emerging roles for intersectin (ITSN) in regulating signaling and disease pathways.International journal of molecular sciences · 2013Review
- Ras1 acts through duplicated Cdc42 and Rac proteins to regulate morphogenesis and pathogenesis in the human fungal pathogen Cryptococcus neoformans.PLoS genetics · 2013Article
- Cryptococcus neoformans phospholipase B1 activates host cell Rac1 for traversal across the blood-brain barrier.Cellular microbiology · 2012Article
- Ras-Mediated Signal Transduction and Virulence in Human Pathogenic Fungi.Fungal genomics & biology · 2012Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Human Wiskott-Aldrich syndrome protein (WASP) is a scaffold linking upstream signals to the actin cytoskeleton. In response to intersectin ITSN1 and Rho GTPase Cdc42, WASP activates the Arp2/3 complex to promote actin polymerization. The human pathogen Cryptococcus neoformans contains the ITSN1 homolog Cin1 and the WASP homolog Wsp1, which share more homology with human proteins than those of other fungi. Here we demonstrate that Cin1, Cdc42/Rac1, and Wsp1 function in an effector pathway similar to that of mammalian models. In the cin1 mutant, expression of the autoactivated Wsp1-B-GBD allele partially suppressed the mutant defect in endocytosis, and expression of the constitutively active CDC42(Q61L) allele restored normal actin cytoskeleton structures. Similar phenotypic suppression can be obtained by the expression of a Cdc42-green fluorescent protein (GFP)-Wsp1 fusion protein. In addition, Rac1, which was found to exhibit a role in early endocytosis, activates Wsp1 to regulate vacuole fusion. Rac1 interacted with Wsp1 and depended on Wsp1 for its vacuolar membrane localization. Expression of the Wsp1-B-GBD allele restored vacuolar membrane fusion in the rac1 mutant. Collectively, our studies suggest novel ways in which this pathogenic fungus has adapted conserved signaling pathways to control vesicle transport and actin organization, likely benefiting survival within infected hosts.
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