Evidence map›Paper›PMID 22326845›Full record

ArticleJournal of pharmaceutical and biomedical analysis2012

A homogeneous fluorescent sensor for human serum albumin.

Rongsheng E Wang, Ling Tian, Yie-Hwa Chang

Abstract readValidation Study
In one paragraph

Article in Journal of pharmaceutical and biomedical analysis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. QCM-based assay designs for human serum albumin.Analytical and bioanalytical chemistry · 2022
    Article
  8. Review
  9. Dual luminescent charge transfer probe for quantitative detection of serum albumin in aqueous samples.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2020
    Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. A rapid, low-cost quantitative diagnostic method for hepatitis C virus infection using capillary zone electrophoresis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014
    Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Rongsheng E WangMediomics, LLC, 5445 Highland Park Drive, St. Louis, MO 63110, USA.
Ling Tian
Yie-Hwa Chang
Mediomics (United States) · USHighland Community College - Illinois · US

Funding

Rapid homogeneous antibody-based detection of proteinsR42GM079891 · NIGMS · MEDIOMICS, LLC · PI HEYDUK, TOMASZ · 2009 to 2010
$1.0M
Rapid homogeneous antibody-based detection of proteinsR41GM079891 · NIGMS · MEDIOMICS, LLC · PI HEYDUK, TOMASZ · 2007 to 2007
$129k
NIGMS NIH HHS GM079891NIGMS NIH HHS GM079891-02S1NIGMS NIH HHS R41 GM079891NIGMS NIH HHS R42 GM079891
6 · The paper itself

Abstract

Human serum albumin is the most abundant protein in the body and is an important biomarker used for disease-related diagnosis. Although the traditional enzyme-linked immunosorbent assay (ELISA) approach can precisely measure the concentration of human serum albumin, the multi-step procedure and time-consuming preparations of ELISA limit its diagnostic applications, preventing accurate point-of-care testing, for example. Herein, we report the recent development of an antibody-based albumin sensor that allows for a homogeneous measurement of albumin concentrations in saliva, urine and serum, in which this type of sensor is validated for the first time. The assay only requires simple mixing, and relies on time-resolved (TR) fluorescence resonance energy transfer (FRET) to produce robust, sensitive signals. The whole process, from sample preparation to final read-out, is expected to take less than 1h and requires only a standard plate-reader, thus making the sensor a convenient and cost-effective tool for albumin analysis.

Indexed as

Biosensing TechniquesFluorescence Resonance Energy TransferAntibodiesAntibody SpecificityBiomarkersEnzyme-Linked Immunosorbent AssayFemaleHumansMaleReproducibility of ResultsSalivaSerum AlbuminAntibodiesBiomarkersSerum Albumin

Identifiers

PMID22326845
PMCPMC3299827
OpenAlexW2054012073

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.