ArticleMethods in cell biology2012
Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding.
Article in Methods in cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Identifying Biological and Biophysical Features of Different Maturation States of α-Synuclein Amyloid Fibrils.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Spatial and Temporal Aspects of Exocytosis Studied on the Isolated Plasma Membranes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Dynamic instability of clathrin assembly provides proofreading control for endocytosis.The Journal of cell biology · 2019Article
- Early Stage Alpha-Synuclein Amyloid Fibrils are Reservoirs of Membrane-Binding Species.Scientific reports · 2019Article
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2 authors.
Funding
Abstract
Cell-free assays represent an important complement to studies in living cells for the elucidation of mechanisms underlying the dynamics of biological membranes, such as budding, fission, and fusion reactions. Here we describe a method for the reconstitution of endocytosis, the process through which cells internalize portions of the plasma membrane along with extracellular material, under conditions that allow the visualization of individual budding events with high spatial and temporal resolution. The method, which is based on the generation of planar plasma membrane sheets attached to a glass substrate and their subsequent incubation with a cytosolic extract, results in a very robust formation of endocytic buds, which upon appropriate conditions undergo fission. The synchronization of the endocytic events and the accessibility of the material to a variety of manipulations make this experimental system a powerful tool for the molecular dissection of endocytosis.
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