ArticleBiophysical journal2012
Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Spatial triple-correlation spectroscopy reveals heterotrimer dynamics in live cells.Biophysical journal · 2026Article
- Pair correlation microscopy of intracellular molecular transport.Nature protocols · 2025Review
- Elucidating the Oligomerization and Cellular Interactions of a Trimer Derived from Aβ through Fluorescence and Mass Spectrometric Studies.ACS chemical neuroscience · 2022Article
- A reappraisal of the form - function problem. Theory and phenomenology.Theory in biosciences = Theorie in den Biowissenschaften · 2022Article
- Ras isoform-specific expression, chromatin accessibility, and signaling.Biophysical reviews · 2021Review
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Unraveling the molecular interactions involved in phase separation of glucocorticoid receptor.BMC biology · 2020Article
- Stability of cytoplasmic nanoviscosity during cell cycle of HeLa cells synchronized with Aphidicolin.Scientific reports · 2019Article
- Characterizing proteins in their cellular environment: Examples of recent advances in quantitative fluorescence microscopy.Protein science : a publication of the Protein Society · 2019Review
- Into the Fourth Dimension: Dysregulation of Genome Architecture in Aging and Alzheimer's Disease.Frontiers in molecular neuroscience · 2018Review
- Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions.Biomedical optics express · 2018Article
- Transcriptional Output Transiently Spikes Upon Mitotic Exit.Scientific reports · 2017Article
- Time-resolved biophysical approaches to nucleocytoplasmic transport.Computational and structural biotechnology journal · 2017Review
- Pair correlation microscopy reveals the role of nanoparticle shape in intracellular transport and site of drug release.Nature nanotechnology · 2017Article
- ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing.Nature methods · 2016Article
- Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells.Biophysical journal · 2016Article
- Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness.Nature communications · 2016Article
- Spatiotemporal regulation of Heterochromatin Protein 1-alpha oligomerization and dynamics in live cells.Scientific reports · 2015Article
- Spatial heterogeneity of dynamics of H1 linker histone.European biophysics journal : EBJ · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We present a quantitative fluctuation-based assay to measure the degree of local chromatin compaction and investigate how chromatin density regulates the diffusive path adopted by an inert protein in dividing cells. The assay uses CHO-K1 cells coexpressing untagged enhanced green fluorescent protein (EGFP) and histone H2B tagged mCherry. We measure at the single-cell level the EGFP localization and molecular flow patterns characteristic of each stage of chromatin compaction from mitosis through interphase by means of pair-correlation analysis. We find that the naturally occurring changes in chromatin organization impart a regulation on the spatial distribution and temporal dynamics of EGFP within the nucleus. Combined with the analysis of Ca(2+) intracellular homeostasis during cell division, EGFP flow regulation can be interpreted as the result of controlled changes in chromatin compaction. For the first time, to our knowledge, we were able to probe chromatin compaction on the micrometer scale, where the regulation of molecular diffusion may become relevant for many cellular processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.