ArticleBiophysical journal2012
Contractile equilibration of single cells to step changes in extracellular stiffness.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- How dynamic prestress governs the shape of living systems, from the subcellular to tissue scale.Interface focus · 2022Review
- Computational models of migration modes improve our understanding of metastasis.APL bioengineering · 2020Review
- Stem Cell Mechanobiology and the Role of Biomaterials in Governing Mechanotransduction and Matrix Production for Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Mechanical Cues Affect Migration and Invasion of Cells From Three Different Directions.Frontiers in cell and developmental biology · 2020Review
- The Functional Response of Mesenchymal Stem Cells to Electron-Beam Patterned Elastomeric Surfaces Presenting Micrometer to Nanoscale Heterogeneous Rigidity.Advanced materials (Deerfield Beach, Fla.) · 2017Article
- Stem cell mechanical behaviour modelling: substrate's curvature influence during adhesion.Biomechanics and modeling in mechanobiology · 2017Article
- Shear-wave elasticity measurements of three-dimensional cell cultures for mechanobiology.Journal of cell science · 2017Article
- Cells as liquid motors: mechanosensitivity emerges from collective dynamics of actomyosin cortex.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Tensional homeostasis in single fibroblasts.Biophysical journal · 2014Article
- A time-dependent phenomenological model for cell mechano-sensing.Biomechanics and modeling in mechanobiology · 2014Article
- Article
- Cells actively stiffen fibrin networks by generating contractile stress.Biophysical journal · 2013Article
- To pull or be pulled: parsing the multiple modes of mechanotransduction.Current opinion in cell biology · 2013Review
- The mechanical environment modulates intracellular calcium oscillation activities of myofibroblasts.PloS one · 2013Article
- Cell mechanics: principles, practices, and prospects.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular stiffness has been shown to alter long timescale cell behaviors such as growth and differentiation, but the cellular response to changes in stiffness on short timescales is poorly understood. By studying the contractile response of cells to dynamic stiffness conditions using an atomic force microscope, we observe a seconds-timescale response to a step change in extracellular stiffness. Specifically, we observe acceleration in contraction velocity (μm/min) and force rate (nN/min) upon a step decrease in stiffness and deceleration upon a step increase in stiffness. Interestingly, this seconds-timescale response to a change in extracellular stiffness is not altered by inhibiting focal adhesion signaling or stretch-activated ion channels and is independent of cell height and contraction force. Rather, the response timescale is altered only by disrupting cytoskeletal mechanics and is well described by a simple mechanical model of a constant velocity actuator pulling against an internal cellular viscoelastic network. Consistent with the predictions of this model, we find that an osmotically expanding hydrogel responds to step changes in extracellular stiffness in a similar manner to cells. We therefore propose that an initial event in stiffness sensing is establishment of a mechanical equilibrium that balances contraction of the viscoelastic cytoskeleton with deformation of the extracellular matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.