ArticleBiophysical journal2012
A genetic strategy for the dynamic and graded control of cell mechanics, motility, and matrix remodeling.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- Role of Microenvironment in Glioma Invasion: What We Learned from In Vitro Models.International journal of molecular sciences · 2018Review
- Activation of ROCK and MLCK tunes regional stress fiber formation and mechanics via preferential myosin light chain phosphorylation.Molecular biology of the cell · 2017Article
- Dynamics of Mechanosensitive Neural Stem Cell Differentiation.Stem cells (Dayton, Ohio) · 2017Article
- Synthetic mechanobiology: engineering cellular force generation and signaling.Current opinion in biotechnology · 2016Review
- Aligned Nanotopography Promotes a Migratory State in Glioblastoma Multiforme Tumor Cells.Scientific reports · 2016Article
- A synthetic hydrogel for the high-throughput study of cell-ECM interactions.Nature communications · 2015Article
- Differential Contributions of Nonmuscle Myosin II Isoforms and Functional Domains to Stress Fiber Mechanics.Scientific reports · 2015Article
- Myosin II Activity Softens Cells in Suspension.Biophysical journal · 2015Article
- Constitutive activation of myosin-dependent contractility sensitizes glioma tumor-initiating cells to mechanical inputs and reduces tissue invasion.Cancer research · 2015Article
- Engineering strategies to mimic the glioblastoma microenvironment.Advanced drug delivery reviews · 2014Review
- Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Materials as stem cell regulators.Nature materials · 2014Review
- Three-dimensional patterning of multiple cell populations through orthogonal genetic control of cell motility.Soft matter · 2014Article
- Matrix identity and tractional forces influence indirect cardiac reprogramming.Scientific reports · 2013Article
- A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.Chemical communications (Cambridge, England) · 2013Article
- Transforming potential and matrix stiffness co-regulate confinement sensitivity of tumor cell migration.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Vinculin tension distributions of individual stress fibers within cell-matrix adhesions.Journal of cell science · 2013Article
- Cellular and Molecular Bioengineering: A Tipping Point.Cellular and molecular bioengineering · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cellular mechanical properties have emerged as central regulators of many critical cell behaviors, including proliferation, motility, and differentiation. Although investigators have developed numerous techniques to influence these properties indirectly by engineering the extracellular matrix (ECM), relatively few tools are available to directly engineer the cells themselves. Here we present a genetic strategy for obtaining graded, dynamic control over cellular mechanical properties by regulating the expression of mutant mechanotransductive proteins from a single copy of a gene placed under a repressible promoter. With the use of constitutively active mutants of RhoA GTPase and myosin light chain kinase, we show that varying the expression level of either protein produces graded changes in stress fiber assembly, traction force generation, cellular stiffness, and migration speed. Using this approach, we demonstrate that soft ECMs render cells maximally sensitive to changes in RhoA activity, and that by modulating the ability of cells to engage and contract soft ECMs, we can dynamically control cell spreading, migration, and matrix remodeling. Thus, in addition to providing quantitative relationships between mechanotransductive signaling, cellular mechanical properties, and dynamic cell behaviors, this strategy enables us to control the physical interactions between cells and the ECM and thereby dictate how cells respond to matrix properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.