ArticleBiophysical journal2012
A hot-spot motif characterizes the interface between a designed ankyrin-repeat protein and its target ligand.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Molecular Docking and Molecular Dynamics Simulations in Related toTropical medicine and infectious disease · 2023Review
- Article
- Protein-protein interaction of RdRp with its co-factor NSP8 and NSP7 to decipher the interface hotspot residues for drug targeting: A comparison between SARS-CoV-2 and SARS-CoV.Journal of molecular structure · 2022Article
- Computational Investigation on the p53-MDM2 Interaction Using the Potential of Mean Force Study.ACS omega · 2020Article
- Molecular Mechanism of Selective Binding of NMS-P118 to PARP-1 and PARP-2: A Computational Perspective.Frontiers in molecular biosciences · 2020Article
- Insight Into the Binding Mechanism of p53/pDIQ-MDMX/MDM2 With the Interaction Entropy Method.Frontiers in chemistry · 2019Article
- Amino acid polymorphisms in the fibronectin-binding repeats of fibronectin-binding protein A affect bond strength and fibronectin conformation.The Journal of biological chemistry · 2017Article
- Ubiquitin-Activated Interaction Traps (UBAITs) identify E3 ligase binding partners.EMBO reports · 2015Article
- Distinct kinetic and mechanical properties govern mucin 16- and podocalyxin-mediated tumor cell adhesion to E- and L-selectin in shear flow.Oncotarget · 2015Article
- Characterization of monobody scaffold interactions with ligand via force spectroscopy and steered molecular dynamics.Scientific reports · 2015Article
- Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase.Biotechnology for biofuels · 2015Article
- Visualization and quantification of protein interactions in the biosynthetic pathway of molybdenum cofactor in Arabidopsis thaliana.Journal of experimental botany · 2013Article
- Distinct kinetic and molecular requirements govern CD44 binding to hyaluronan versus fibrin(ogen).Biophysical journal · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Nonantibody scaffolds such as designed ankyrin repeat proteins (DARPins) can be rapidly engineered to detect diverse target proteins with high specificity and offer an attractive alternative to antibodies. Using molecular simulations, we predicted that the binding interface between DARPin off7 and its ligand (maltose binding protein; MBP) is characterized by a hot-spot motif in which binding energy is largely concentrated on a few amino acids. To experimentally test this prediction, we fused MBP to a transmembrane domain to properly orient the protein into a polymer-cushioned lipid bilayer, and characterized its interaction with off7 using force spectroscopy. Using this, to our knowledge, novel technique along with surface plasmon resonance, we validated the simulation predictions and characterized the effects of select mutations on the kinetics of the off7-MBP interaction. Our integrated approach offers scientific insights on how the engineered protein interacts with the target molecule.
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