ArticleCell proliferation2012
Derivation of male germ cells from induced pluripotent stem cells in vitro and in reconstituted seminiferous tubules.
Article in Cell proliferation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Testicular immunity.Molecular aspects of medicine · 2024Review
- The Evolutionary Route of in vitro Human Spermatogenesis: What is the Next Destination?Stem cell reviews and reports · 2024Review
- Current Progress in Stem Cell Therapy for Male Infertility.Stem cell reviews and reports · 2023Review
- Review
- Male infertility treatment for cancer survivors: does anticancer treatment affect infertility treatment?Nagoya journal of medical science · 2020Article
- Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers.Cell journal · 2019Article
- Recovery of Spermatogenesis Following Cancer Treatment with Cytotoxic Chemotherapy and Radiotherapy.The world journal of men's health · 2019Review
- Spermatogenesis after transplantation of adipose tissue-derived mesenchymal stem cells in busulfan-induced azoospermic hamster.Iranian journal of basic medical sciences · 2018Article
- Recent advances of in vitro culture systems for spermatogonial stem cells in mammals.Reproductive medicine and biology · 2018Review
- Efficient generation of functional haploid spermatids from human germline stem cells by three-dimensional-induced system.Cell death and differentiation · 2018Article
- Research on Human Embryos and Reproductive Materials: Revisiting Canadian Law and Policy.Healthcare policy = Politiques de sante · 2018Article
- Human induced pluripotent stem cells and male infertility: an overview of current progress and perspectives.Human reproduction (Oxford, England) · 2018Review
- Induced pluripotent stem cell: A headway in reprogramming with promising approach in regenerative biology.Veterinary world · 2017Review
- In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells.Stem cell research & therapy · 2017Article
- Induction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamster.International journal of stem cells · 2015Article
- Differentiation of human umbilical cord mesenchymal stem cells into germ-like cells in mouse seminiferous tubules.Molecular medicine reports · 2015Article
- Generation of haploid spermatids with fertilization and development capacity from human spermatogonial stem cells of cryptorchid patients.Stem cell reports · 2014Article
- Stem cells as new agents for the treatment of infertility: current and future perspectives and challenges.BioMed research international · 2014Review
- Induced pluripotent stem cell potential in medicine, specifically focused on reproductive medicine.Frontiers in surgery · 2014Review
- Niemann-Pick type C1 patient-specific induced pluripotent stem cells display disease specific hallmarks.Orphanet journal of rare diseases · 2013Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesPrevious studies have demonstrated that mouse- and human-induced pluripotent stem (iPS) cells can differentiate into primordial germ cells in vitro. However, up to now it is not known whether iPS cells would be able to differentiate into male germ cells in vivo. The aim of this study was to explore differentiation potential of iPS cells to male germ cells in vitro and in vivo. MATERIALS AND
methodsIn this study, approaches using in vitro retinoic acid induction and in vivo ectopic transplantation were combined to induce iPS cells to become male germ cells.
resultsRT-PCR showed that expression of pre-meiotic and meiotic germ cell-specific genes was enhanced in iPS cell-derived embryoid bodies (EBs) compared to mRNA transcripts of iPS cells. Immunofluorescence analysis revealed that iPS cell-derived EBs positively expressed germ-cell markers VASA, c-Kit and SCP3. Furthermore, iPS cell-derived cells dissociated from EBs were injected with testicular cells into dorsal skin of mice. Histological examination showed that iPS cell-derived cells could reconstitute seminiferous tubules, and meanwhile, iPS cell-derived germ cells could settle at basement membranes of reconstituted tubules.
conclusionOur results suggest that iPS cells are able to differentiate into male germ cells in vitro and that reconstituted seminiferous tubules may provide a functional niche for exogenous iPS cell-derived male germ cells. Derivation of male germ cells from iPS cells has potential application for treating male infertility and provides an ideal platform for elucidating molecular mechanisms of male germ-cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.