ArticlePlant physiology2012
Transport of indole-3-butyric acid and indole-3-acetic acid in Arabidopsis hypocotyls using stable isotope labeling.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 46 citations in OpenAlex.
- Indole-3-Butyric Acid (IBA) Derivatives for Root Architecture Modulation: Potent Promoters viaACS omega · 2025Article
- Factors governing cellular reprogramming competence in Arabidopsis adventitious root formation.Developmental cell · 2024Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- Transcriptome Dynamics of Rooting Zone and Leaves during In Vitro Adventitious Root Formation inPlants (Basel, Switzerland) · 2022Article
- Auxin Metabolism in Plants.Cold Spring Harbor perspectives in biology · 2021Review
- OsIAGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Auxins in Rice.Rice (New York, N.Y.) · 2019Article
- ABCG36/PEN3/PDR8 Is an Exporter of the Auxin Precursor, Indole-3-Butyric Acid, and Involved in Auxin-Controlled Development.Frontiers in plant science · 2019Article
- Indole 3-Butyric Acid Metabolism and Transport inFrontiers in plant science · 2019Review
- Out of Shape During Stress: A Key Role for Auxin.Trends in plant science · 2018Review
- Roles for IBA-derived auxin in plant development.Journal of experimental botany · 2018Review
- Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.Plant cell reports · 2017Article
- Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity.BMC plant biology · 2017Article
- Ethylene and auxin interaction in the control of adventitious rooting in Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Connective Auxin Transport in the Shoot Facilitates Communication between Shoot Apices.PLoS biology · 2016Article
- Ectopic expression of UGT75D1, a glycosyltransferase preferring indole-3-butyric acid, modulates cotyledon development and stress tolerance in seed germination of Arabidopsis thaliana.Plant molecular biology · 2016Article
- The Control of Auxin Transport in Parasitic and Symbiotic Root-Microbe Interactions.Plants (Basel, Switzerland) · 2015Review
- Arabidopsis SHR and SCR transcription factors and AUX1 auxin influx carrier control the switch between adventitious rooting and xylogenesis in planta and in in vitro cultured thin cell layers.Annals of botany · 2015Article
- Auxin activity: Past, present, and future.American journal of botany · 2015Review
- Article
- Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples.Plant methods · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers. Because radioactive assays alone cannot distinguish IBA from its metabolites, the detected transport from applied [3H]IBA may have resulted from the transport of IBA metabolites, including IAA. To test this hypothesis, we used a mass spectrometry-based method to quantify the transport of IBA in Arabidopsis hypocotyls by following the movement of [13C1]IBA and the [13C1]IAA derived from [13C1]IBA. We also assayed [13C6]IAA transport in a parallel control experiment. We found that the amount of transported [13C1]IBA was dramatically lower than [13C6]IAA, and the IBA transport was not reduced by the auxin transport inhibitor N-1-naphthylphthalamic acid. Significant amounts of the applied [13C1]IBA were converted to [13C1]IAA during transport, but [13C1]IBA transport was independent of IBA-to-IAA conversion. We also found that most of the [13C1]IBA was converted to ester-linked [13C1]IBA at the apical end of hypocotyls, and ester-linked [13C1]IBA was also found in the basal end at a level higher than free [13C1]IBA. In contrast, most of the [13C6]IAA was converted to amide-linked [13C6]IAA at the apical end of hypocotyls, but very little conjugated [13C6]IAA was found in the basal end. Our results demonstrate that the polar transport of IBA is much lower than IAA in Arabidopsis hypocotyls, and the transport mechanism is distinct from IAA transport. These experiments also establish a method for quantifying the movement of small molecules in plants using stable isotope labeling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.