ArticleMolecular biology of the cell2012
The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
- Alterations in lipid saturation trigger remodeling of the outer mitochondrial membrane.Molecular biology of the cell · 2025Article
- Nitrogen limitation causes a seismic shift in redox state and phosphorylation of proteins implicated in carbon flux and lipidome remodeling in Rhodotorula toruloides.Biotechnology for biofuels and bioproducts · 2025Article
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Targeting fungal lipid synthesis for antifungal drug development and potentiation of contemporary antifungals.npj antimicrobials and resistance · 2025Review
- Temperature adaptation of yeast phospholipid molecular species at the acyl chain positional level.FEBS letters · 2025Article
- Lipid saturation controls nuclear envelope function.Nature cell biology · 2023Article
- Molecular species selectivity of lipid transport creates a mitochondrial sink for di-unsaturated phospholipids.The EMBO journal · 2022Article
- Enhancing very long chain fatty acids production in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Engineering of Saccharomyces cerevisiae for the accumulation of high amounts of triacylglycerol.Microbial cell factories · 2021Article
- Phosphatidic acid biosynthesis in the model organism yeast Saccharomyces cerevisiae - a survey.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Review
- Multifunctional cationic surfactants with a labile amide linker as efficient antifungal agents-mechanisms of action.Applied microbiology and biotechnology · 2021Article
- The topology of the ER-resident phospholipid methyltransferase Opi3 ofThe Journal of biological chemistry · 2020Article
- Phospholipid turnover and acyl chain remodeling in the yeast ER.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Dysregulation of very-long-chain fatty acid metabolism causes membrane saturation and induction of the unfolded protein response.Molecular biology of the cell · 2020Article
- Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment.Molecular cell · 2019Article
- The glycerophosphocholine acyltransferase Gpc1 is part of a phosphatidylcholine (PC)-remodeling pathway that alters PC species in yeast.The Journal of biological chemistry · 2019Article
- The role of phospholipid molecular species in determining the physical properties of yeast membranes.FEBS letters · 2018Review
- Errors in protein synthesis increase the level of saturated fatty acids and affect the overall lipid profiles of yeast.PloS one · 2018Article
- Glycerol conversion into a single cell oil by engineeredEngineering in life sciences · 2017Article
- 1,2,4-Triazolidine-3-thiones Have Specific Activity against Acinetobacter baumannii among Common Nosocomial Pathogens.ACS infectious diseases · 2017Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The degree of fatty acid unsaturation, that is, the ratio of unsaturated versus saturated fatty acyl chains, determines membrane fluidity. Regulation of expression of the fatty acid desaturase Ole1p was hitherto the only known mechanism governing the degree of fatty acid unsaturation in Saccharomyces cerevisiae. We report a novel mechanism for the regulation of fatty acid desaturation that is based on competition between Ole1p and the glycerol-3-phosphate acyltransferase Sct1p/Gat2p for the common substrate C16:0-CoA. Deletion of SCT1 decreases the content of saturated fatty acids, whereas overexpression of SCT1 dramatically decreases the desaturation of fatty acids and affects phospholipid composition. Whereas overexpression of Ole1p increases desaturation, co-overexpression of Ole1p and Sct1p results in a fatty acid composition intermediate between those obtained upon overexpression of the enzymes separately. On the basis of these results, we propose that Sct1p sequesters C16:0-CoA into lipids, thereby shielding it from desaturation by Ole1p. Ta-king advantage of the growth defect conferred by overexpressing SCT1, we identified the acyltransferase Cst26p/Psi1p as a regulator of Sct1p activity by affecting the phosphorylation state and overexpression level of Sct1p. The level of Sct1p phosphorylation is increased when cells are supplemented with saturated fatty acids, demonstrating the physiological relevance of our findings.
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