ArticleMolecular biology of the cell2012
FCH domain only-2 organizes clathrin-coated structures and interacts with Disabled-2 for low-density lipoprotein receptor endocytosis.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Cargo-selective regulation of clathrin-mediated endocytosis by AMP-activated protein kinase.iScience · 2025Article
- Multivariate investigation of aging in mouse models expressing the Alzheimer's protective APOE2 allele: integrating cognitive metrics, brain imaging, and blood transcriptomics.Brain structure & function · 2024Article
- FCHO controls AP2's initiating role in endocytosis through a PtdIns(4,5)PScience advances · 2022Article
- Immune microenvironment-related gene mapping predicts immunochemotherapy response and prognosis in diffuse large B-cell lymphoma.Medical oncology (Northwood, London, England) · 2022Article
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- Inclusion of endophenotypes in a standard GWAS facilitate a detailed mechanistic understanding of genetic elements that control blood lipid levels.Scientific reports · 2020Article
- DNA methylation microarrays identify epigenetically regulated lipid related genes in obese patients with hypercholesterolemia.Molecular medicine (Cambridge, Mass.) · 2020Article
- Dab2 gene variant is associated with increased coronary artery disease risk in Chinese Han population.Medicine · 2020Article
- Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells.Nature communications · 2020Article
- Endocytosis and Organelle Targeting of Nanomedicines in Cancer Therapy.International journal of nanomedicine · 2020Review
- Nanoscale coupling of endocytic pit growth and stability.Science advances · 2019Article
- Endocytic Adaptor Proteins in Health and Disease: Lessons from Model Organisms and Human Mutations.Cells · 2019Review
- Conformational regulation of AP1 and AP2 clathrin adaptor complexes.Traffic (Copenhagen, Denmark) · 2019Review
- Emerging concepts of receptor endocytosis and concurrent intracellular signaling: Mechanisms of guanylyl cyclase/natriuretic peptide receptor-A activation and trafficking.Cellular signalling · 2019Review
- Syp1 regulates the clathrin-mediated and clathrin-independent endocytosis of multiple cargo proteins through a novel sorting motif.Molecular biology of the cell · 2017Article
- Endocytosis and membrane receptor internalization: implication of F-BAR protein Carom.Frontiers in bioscience (Landmark edition) · 2017Review
- Transient Fcho1/2⋅Eps15/R⋅AP-2 Nanoclusters Prime the AP-2 Clathrin Adaptor for Cargo Binding.Developmental cell · 2016Article
- Structural basis for the recognition of two consecutive mutually interacting DPF motifs by the SGIP1 μ homology domain.Scientific reports · 2016Article
- Endocytosis and Physiology: Insights from Disabled-2 Deficient Mice.Frontiers in cell and developmental biology · 2016Review
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
Clathrin-mediated endocytosis regulates the internalization of many nutrient and signaling receptors. Clathrin and endocytic accessory proteins are recruited to receptors by specific adaptors. The adaptor Disabled-2 (Dab2) recruits its cargoes, including the low-density lipoprotein receptor (LDLR), and mediates endocytosis, even when the major adaptor protein AP2 is depleted. We hypothesized that the accessory proteins normally recruited by AP2 may be recruited by Dab2 if AP2 is absent. We identified one such accessory protein, the F-BAR protein FCH domain only-2 (FCHO2), as a major Dab2-interacting protein. The μ-homology domain (μHD) of FCHO2 binds directly to DPF sequences in Dab2 that also bind AP2. Disrupting the Dab2-FCHO2 interaction inhibited Dab2-mediated LDLR endocytosis in AP2-depleted cells. Depleting FCHO2 reduced the number but increased the size of clathrin structures on the adherent surface of HeLa cells and inhibited LDLR and transferrin receptor clustering. However, LDLR was internalized efficiently by FCHO2-deficient cells when additional time was provided for LDLR to enter the enlarged structures before budding, suggesting that later steps of endocytosis are normal under these conditions. These results indicate FCHO2 regulates the size of clathrin structures, and its interaction with Dab2 is needed for LDLR endocytosis under conditions of low AP2.
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