ArticleMalaria journal2012
Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum-infected whole blood samples.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 108 citations in OpenAlex.
- Analysis of anti-malarial resistance markers in pfmdr1 and pfcrt across Southeast Asia in the Tracking Resistance to Artemisinin Collaboration.Malaria journal · 2016Trial
- Genetic architecture of artemisinin-resistant Plasmodium falciparum.Nature genetics · 2015Trial
- Therapeutic efficacy and safety of artemether-lumefantrine for the treatment of uncomplicated falciparum malaria in North-Eastern Tanzania.Malaria journal · 2014Trial
- Population genomics of Plasmodium malariae from 4 African countries.JCI insight · 2026Article
- Hidden hematological, biochemical and immune costs of asymptomatic malaria infections in semi-wild chimpanzees.PLoS pathogens · 2026Article
- Hidden hematological, biochemical and immune costs of asymptomatic malaria infections in semi-wild chimpanzees.bioRxiv : the preprint server for biology · 2025Article
- Low-volumeMicrobial genomics · 2025Article
- Novel single-cell preservation and RNA sequencing technology unlocks field studies for Plasmodium natural infections.Scientific reports · 2025Article
- Genomic analysis of Plasmodium vivax field isolates circulating in sub-Saharan Africa.Communications biology · 2025Article
- Population genomics of Plasmodium ovale species in sub-Saharan Africa.Nature communications · 2024Article
- Population genomics ofbioRxiv : the preprint server for biology · 2024Article
- Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment.PLoS pathogens · 2024Review
- Genetic polymorphism and evidence of signatures of selection in the Plasmodium falciparum circumsporozoite protein gene in Tanzanian regions with different malaria endemicity.Malaria journal · 2024Article
- Genetic polymorphism and evidence of signatures of selection in themedRxiv : the preprint server for health sciences · 2024Article
- Asymptomatic Plasmodium falciparum carriage at the end of the dry season is associated with subsequent infection and clinical malaria in Eastern Gambia.Malaria journal · 2024Article
- Selective whole-genome sequencing ofmBio · 2024Article
- Detection of novelFrontiers in cellular and infection microbiology · 2024Article
- Development and Optimization of a Selective Whole-Genome Amplification To Study Plasmodium ovale Spp.Microbiology spectrum · 2022Article
- 5WBF: a low-cost and straightforward whole blood filtration method suitable for whole-genome sequencing of Plasmodium falciparum clinical isolates.Malaria journal · 2022Article
- RNAseq of Infected Erythrocyte Surface Antigen-Encoding Genes.Methods in molecular biology (Clifton, N.J.) · 2022Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
Abstract
backgroundGenome and transcriptome studies of Plasmodium nucleic acids obtained from parasitized whole blood are greatly improved by depletion of human DNA or enrichment of parasite DNA prior to next-generation sequencing and microarray hybridization. The most effective method currently used is a two-step procedure to deplete leukocytes: centrifugation using density gradient media followed by filtration through expensive, commercially available columns. This method is not easily implemented in field studies that collect hundreds of samples and simultaneously process samples for multiple laboratory analyses. Inexpensive syringes, hand-packed with CF11 cellulose powder, were recently shown to improve ex vivo cultivation of Plasmodium vivax obtained from parasitized whole blood. This study was undertaken to determine whether CF11 columns could be adapted to isolate Plasmodium falciparum DNA from parasitized whole blood and achieve current quantity and purity requirements for Illumina sequencing.
methodsThe CF11 procedure was compared with the current two-step standard of leukocyte depletion using parasitized red blood cells cultured in vitro and parasitized blood obtained ex vivo from Cambodian patients with malaria. Procedural variations in centrifugation and column size were tested, along with a range of blood volumes and parasite densities.
resultsCF11 filtration reliably produces 500 nanograms of DNA with less than 50% human DNA contamination, which is comparable to that obtained by the two-step method and falls within the current quality control requirements for Illumina sequencing. In addition, a centrifuge-free version of the CF11 filtration method to isolate P. falciparum DNA at remote and minimally equipped field sites in malaria-endemic areas was validated.
conclusionsCF11 filtration is a cost-effective, scalable, one-step approach to remove human DNA from P. falciparum-infected whole blood samples.
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