ArticleMolecular microbiology2012
The ancillary protein 1 of Streptococcus pyogenes FCT-1 pili mediates cell adhesion and biofilm formation through heterophilic as well as homophilic interactions.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Group A Streptococcus Pili-Roles in Pathogenesis and Potential for Vaccine Development.Microorganisms · 2024Review
- Identification of an immunodominant region on a group AVirulence · 2023Article
- Clostridium perfringens Produces an Adhesive Pilus Required for the Pathogenesis of Necrotic Enteritis in Poultry.Journal of bacteriology · 2021Article
- Genetics, Structure, and Function of Group A Streptococcal Pili.Frontiers in microbiology · 2021Review
- Deciphering Streptococcal Biofilms.Microorganisms · 2020Review
- Streptococcus pyogenes Capsule Promotes Microcolony-Independent Biofilm Formation.Journal of bacteriology · 2019Article
- Current Understanding of Group A Streptococcal Biofilms.Current drug targets · 2019Review
- PilVax - a novel peptide delivery platform for the development of mucosal vaccines.Scientific reports · 2018Article
- Mucosal vaccination with pili from Group A Streptococcus expressed on Lactococcus lactis generates protective immune responses.Scientific reports · 2017Article
- Novel Molecular Insights about Lactobacillar Sortase-Dependent Piliation.International journal of molecular sciences · 2017Review
- Tissue tropisms in group A Streptococcus: what virulence factors distinguish pharyngitis from impetigo strains?Current opinion in infectious diseases · 2016Review
- Streptococcus pyogenes biofilms-formation, biology, and clinical relevance.Frontiers in cellular and infection microbiology · 2015Review
- Article
- Pilin and sortase residues critical for endocarditis- and biofilm-associated pilus biogenesis in Enterococcus faecalis.Journal of bacteriology · 2013Article
- Article
- The small regulatory RNA FasX controls pilus expression and adherence in the human bacterial pathogen group A Streptococcus.Molecular microbiology · 2012Article
- Pilus biogenesis in Lactococcus lactis: molecular characterization and role in aggregation and biofilm formation.PloS one · 2012Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gram-positive pili are known to play a role in bacterial adhesion to epithelial cells and in the formation of biofilm microbial communities. In the present study we undertook the functional characterization of the pilus ancillary protein 1 (AP1_M6) from Streptococcus pyogenes isolates expressing the FCT-1 pilus variant, known to be strong biofilm formers. Cell binding and biofilm formation assays using S. pyogenes in-frame deletion mutants, Lactococcus expressing heterologous FCT-1 pili and purified recombinant AP1_M6, indicated that this pilin is a strong cell adhesin that is also involved in bacterial biofilm formation. Moreover, we show that AP1_M6 establishes homophilic interactions that mediate inter-bacterial contact, possibly promoting bacterial colonization of target epithelial cells in the form of three-dimensional microcolonies. Finally, AP1_M6 knockout mutants were less virulent in mice, indicating that this protein is also implicated in GAS systemic infection.
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