ArticleSpermatogenesis2011
Testin and actin are key molecular targets of adjudin, an anti-spermatogenic agent, in the testis.
Article in Spermatogenesis, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Advances in Male Contraception: When Will the Novel Male Contraception be Available?The world journal of men's health · 2024Review
- Aquaporin 4 in Traumatic Brain Injury: From Molecular Pathways to Therapeutic Target.Neurochemical research · 2022Review
- Review
- Emerging approaches to male contraception.Fertility and sterility · 2021Review
- Signaling Proteins That Regulate Spermatogenesis Are the Emerging Target of Toxicant-Induced Male Reproductive Dysfunction.Frontiers in endocrinology · 2021Review
- Development of Novel Male Contraceptives.Clinical and translational science · 2020Review
- Toward Development of the Male Pill: A Decade of Potential Non-hormonal Contraceptive Targets.Frontiers in cell and developmental biology · 2020Review
- Dynein 1 supports spermatid transport and spermiation during spermatogenesis in the rat testis.American journal of physiology. Endocrinology and metabolism · 2018Article
- Male contraception.Fertility and sterility · 2016Review
- Regulation of microtubule (MT)-based cytoskeleton in the seminiferous epithelium during spermatogenesis.Seminars in cell & developmental biology · 2016Review
- Beyond the Condom: Frontiers in Male Contraception.Seminars in reproductive medicine · 2016Review
- Effective Delivery of Male Contraceptives Behind the Blood-Testis Barrier (BTB) - Lesson from Adjudin.Current medicinal chemistry · 2016Review
- Interaction of oligomeric breast cancer resistant protein (BCRP) with adjudin: a male contraceptive with anti-cancer activity.Current molecular pharmacology · 2014Review
- The apical ES-BTB-BM functional axis is an emerging target for toxicant-induced infertility.Trends in molecular medicine · 2013Article
- New approaches to male non-hormonal contraception.Contraception · 2013Review
- Inhibition of sperm capacitation and fertilizing capacity by adjudin is mediated by chloride and its channels in humans.Human reproduction (Oxford, England) · 2013Article
- Testin and its emerging modulatory role in systemic carcinogenesis.Spermatogenesis · 2012Article
- Rat and mouse testicular testin is different from the human tumor suppressor gene TESTIN (Tes): Authors' response to the letter of Dr. S. Kapoor.Spermatogenesis · 2012Article
- Adjudin disrupts spermatogenesis via the action of some unlikely partners: Eps8, Arp2/3 complex, drebrin E, PAR6 and 14-3-3.Spermatogenesis · 2011Article
- Interactions of laminin β3 fragment with β1-integrin receptor: A revisit of the apical ectoplasmic specialization-blood-testis-barrier-hemidesmosome functional axis in the testis.Spermatogenesis · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Earlier studies have shown adjudin to cause aspermatogenesis by depleting virtually all germ cells from the seminiferous epithelium, leading to transient infertility; spermatogenesis and fertility were re-established several weeks later after germ cell proliferation and differentiation were reinitiated by spermatogonia. While adjudin is known to exert its initial effects at the apical ectoplasmic specialization (a testis-specific atypical anchoring junction), thereby perturbing spermatid adhesion, its molecular target(s) at this site is not known. Herein, we report the production of a specific antibody against adjudin after this compound was conjugated to an adjuvant (i.e., keyhole limpet hemocyanin) to maximize immune response in rabbits. This antibody was utilized for co-immunoprecipitation by using an affinity resin to pull-down the binding partners of adjudin. Using this approach coupled with mass spectrometry and immunoblotting, we show testin (a protein largely restricted to the apical ES in the adult testis) and actin-myosin to be molecular targets of adjudin. These findings provide a platform for future functional studies, not only to better understand the molecular mechanism behind adjudin-induced germ cell loss from the seminiferous epithelium, but also to understand the molecular basis of spermiation.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.