ArticlePLoS genetics2012
Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Spindle integrity is regulated by a phospho-dependent interaction between the Ndc80 and Dam1 kinetochore complexes.PLoS genetics · 2025Article
- Evidence of 14-3-3 proteins contributing to kinetochore integrity and chromosome congression during mitosis.Journal of cell science · 2024Article
- Role of spindle assembly checkpoint proteins in gametogenesis and embryogenesis.Frontiers in cell and developmental biology · 2024Review
- A new layer of regulation of chromosomal passenger complex (CPC) translocation in budding yeast.Molecular biology of the cell · 2023Article
- Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast.Molecular biology of the cell · 2023Article
- Article
- Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.PLoS genetics · 2021Article
- The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.The Journal of cell biology · 2020Article
- Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast.PLoS genetics · 2020Article
- Article
- The Opposing Functions of Protein Kinases and Phosphatases in Chromosome Bipolar Attachment.International journal of molecular sciences · 2019Review
- A Gradient in Metaphase Tension Leads to a Scaled Cellular Response in Mitosis.Developmental cell · 2019Article
- The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast.Scientific reports · 2017Article
- Premature Silencing of the Spindle Assembly Checkpoint Is Prevented by the Bub1-H2A-Sgo1-PP2A Axis inGenetics · 2017Article
- Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase.Cell reports · 2016Article
- Candida albicans Kinesin Kar3 Depends on a Cik1-Like Regulatory Partner Protein for Its Roles in Mating, Cell Morphogenesis, and Bipolar Spindle Formation.Eukaryotic cell · 2015Article
- Sgo1 regulates both condensin and Ipl1/Aurora B to promote chromosome biorientation.PLoS genetics · 2014Article
- The current view for the silencing of the spindle assembly checkpoint.Cell cycle (Georgetown, Tex.) · 2014Review
- The signaling network that silences the spindle assembly checkpoint upon the establishment of chromosome bipolar attachment.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Slk19 clusters kinetochores and facilitates chromosome bipolar attachment.Molecular biology of the cell · 2013Article
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5 authors.
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Abstract
The attachment of sister kinetochores by microtubules emanating from opposite spindle poles establishes chromosome bipolar attachment, which generates tension on chromosomes and is essential for sister-chromatid segregation. Syntelic attachment occurs when both sister kinetochores are attached by microtubules from the same spindle pole and this attachment is unable to generate tension on chromosomes, but a reliable method to induce syntelic attachments is not available in budding yeast. The spindle checkpoint can sense the lack of tension on chromosomes as well as detached kinetochores to prevent anaphase onset. In budding yeast Saccharomyces cerevisiae, tension checkpoint proteins Aurora/Ipl1 kinase and centromere-localized Sgo1 are required to sense the absence of tension but are dispensable for the checkpoint response to detached kinetochores. We have found that the loss of function of a motor protein complex Cik1/Kar3 in budding yeast leads to syntelic attachments. Inactivation of either the spindle or tension checkpoint enables premature anaphase entry in cells with dysfunctional Cik1/Kar3, resulting in co-segregation of sister chromatids. Moreover, the abolished Kar3-kinetochore interaction in cik1 mutants suggests that the Cik1/Kar3 complex mediates chromosome movement along microtubules, which could facilitate bipolar attachment. Therefore, we can induce syntelic attachments in budding yeast by inactivating the Cik1/Kar3 complex, and this approach will be very useful to study the checkpoint response to syntelic attachments.
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