ArticleNucleic acids research2012
Analysis of MRE11's function in the 5'-->3' processing of DNA double-strand breaks.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Resection of DNA double-strand breaks activates Mre11-Rad50-Nbs1- and Rad9-Hus1-Rad1-dependent mechanisms that redundantly promote ATR checkpoint activation and end processing in Xenopus egg extracts.Nucleic acids research · 2024Article
- MRN-dependent and independent pathways for recruitment of TOPBP1 to DNA double-strand breaks.PloS one · 2022Article
- Structure-function analysis of TOPBP1's role in ATR signaling using the DSB-mediated ATR activation in Xenopus egg extracts (DMAX) system.Scientific reports · 2021Article
- Ubiquitin and ubiquitin-like molecules in DNA double strand break repair.Cell & bioscience · 2020Review
- A Drosophila cell-free system that senses DNA breaks and triggers phosphorylation signalling.Nucleic acids research · 2019Article
- Regulatory control of Sgs1 and Dna2 during eukaryotic DNA end resection.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Dna2 initiates resection at clean DNA double-strand breaks.Nucleic acids research · 2017Article
- Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.Methods in enzymology · 2017Article
- The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair.Nucleic acids research · 2016Article
- Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.Nucleic acids research · 2016Article
- The N-terminus of RPA large subunit and its spatial position are important for the 5'->3' resection of DNA double-strand breaks.Nucleic acids research · 2015Article
- DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end resection in human cells.The Journal of biological chemistry · 2014Article
- DNA double-strand break repair pathway choice and cancer.DNA repair · 2014Review
- PCNA promotes processive DNA end resection by Exo1.Nucleic acids research · 2013Article
- Microhomology-mediated End Joining and Homologous Recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Activation of DSB processing requires phosphorylation of CtIP by ATR.Molecular cell · 2013Article
- End-resection at DNA double-strand breaks in the three domains of life.Biochemical Society transactions · 2013Review
- Distinct functions of human RECQ helicases WRN and BLM in replication fork recovery and progression after hydroxyurea-induced stalling.DNA repair · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The resection of DNA double-strand breaks (DSBs) into 3' single-strand tails is the initiating step of homology-dependent repair pathways. A key player in this process is the MRE11-RAD50-NBS1 complex, but its contribution to and mechanistic role in resection are not well understood. In this study, we took advantage of the Xenopus egg extract system to address these questions. We found that depletion of MRE11 caused a dramatic inhibition of 5'-resection, even for the first nucleotide at the 5'-end. Depletion of Xenopus CtIP also inhibited 5'-strand resection, but this inhibition could be alleviated by excess MRN. Both MRE11 and CtIP could be bypassed by a DNA that carried a 3'-ss-tail. Finally, using purified proteins, we found that MRN could stimulate both the WRN-DNA2-RPA pathway and the EXO1 pathway of resection. These findings provide important insights into the function of MRE11 in 5'-strand resection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.