ArticlePlant physiology2012
A peroxidase-dependent apoplastic oxidative burst in cultured Arabidopsis cells functions in MAMP-elicited defense.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 1 of them a synthesis that pooled it.
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Who cites it
97 citing papers in PubMed, 1 synthesis or guideline pooled it, 228 citations in OpenAlex.
- Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks.International journal of molecular sciences · 2018Pooled it
- Integrated omics reveal phenylpropanoid biosynthesis as a key defense pathway against Meloidogyne incognita in Cucumis metuliferus.BMC plant biology · 2026Article
- A novel MLO protein CsMLO4 plays an essential role in cucumber resistance to target leaf spot.Horticulture research · 2025Article
- ARABIDOPSIS HOMOLOG OF TRITHORAX1 impacts lateral root development by epigenetic regulation of targets involved in root system architecture.The New phytologist · 2025Article
- Maize Peroxidase ZmPrx25 Modulates Apoplastic ROS Homeostasis and Promotes Seed Germination and Growth Under Osmotic and Drought Stresses.Antioxidants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification and Characterization of the Class III Peroxidase Gene Family in Radish (International journal of molecular sciences · 2025Article
- Reactive Oxygen Species in Plants: Metabolism, Signaling, and Oxidative Modifications.Antioxidants (Basel, Switzerland) · 2025Review
- Dressed Up to the Nines: The Interplay of Phytohormones Signaling and Redox Metabolism During Plant Response to Drought.Plants (Basel, Switzerland) · 2025Review
- Identification and functional analysis of putative effector proteins fromFrontiers in plant science · 2025Article
- Article
- Cyclo (Pro-Tyr) upregulatesFrontiers in plant science · 2025Article
- Antioxidant Responses and Redox Regulation Within Plant-Beneficial Microbe Interaction.Antioxidants (Basel, Switzerland) · 2024Review
- The molecular dynamics between reactive oxygen species (ROS), reactive nitrogen species (RNS) and phytohormones in plant's response to biotic stress.Plant cell reports · 2024Review
- New Insights into the Connections between Flooding/Hypoxia Response and Plant Defenses against Pathogens.Plants (Basel, Switzerland) · 2024Review
- Genome-wide association analysis reveals genes controlling an antagonistic effect of biotic and osmotic stress on Arabidopsis thaliana growth.Molecular plant pathology · 2024Article
- CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD.Horticulture research · 2024Article
- Unveiling the role of epigenetic mechanisms and redox signaling in alleviating multiple abiotic stress in plants.Frontiers in plant science · 2024Review
- Transcriptional control of hydrogen peroxide homeostasis regulates ground tissue patterning in theFrontiers in plant science · 2023Article
- ROS and RNS Alterations in the Digestive Fluid ofPlants (Basel, Switzerland) · 2022Article
- Knockout of MITOGEN-ACTIVATED PROTEIN KINASE 3 causes barley root resistance against Fusarium graminearum.Plant physiology · 2022Article
37 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Perception by plants of so-called microbe-associated molecular patterns (MAMPs) such as bacterial flagellin, referred to as pattern-triggered immunity, triggers a rapid transient accumulation of reactive oxygen species (ROS). We previously identified two cell wall peroxidases, PRX33 and PRX34, involved in apoplastic hydrogen peroxide (H2O2) production in Arabidopsis (Arabidopsis thaliana). Here, we describe the generation of Arabidopsis tissue culture lines in which the expression of PRX33 and PRX34 is knocked down by antisense expression of a heterologous French bean (Phaseolus vulgaris) peroxidase cDNA construct. Using these tissue culture lines and two inhibitors of ROS generation, azide and diphenylene iodonium, we found that perxoxidases generate about half of the H2O2 that accumulated in response to MAMP treatment and that NADPH oxidases and other sources such as mitochondria account for the remainder of the ROS. Knockdown of PRX33/PRX34 resulted in decreased expression of several MAMP-elicited genes, including MYB51, CYP79B2, and CYP81F2. Similarly, proteomic analysis showed that knockdown of PRX33/PRX34 led to the depletion of various MAMP-elicited defense-related proteins, including the two cysteine-rich peptides PDF2.2 and PDF2.3. Knockdown of PRX33/PRX34 also led to changes in the cell wall proteome, including increases in enzymes involved in cell wall remodeling, which may reflect enhanced cell wall expansion as a consequence of reduced H2O2-mediated cell wall cross-linking. Comparative metabolite profiling of a CaCl2 extract of the PRX33/PRX34 knockdown lines showed significant changes in amino acids, aldehydes, and keto acids but not fatty acids and sugars. Overall, these data suggest that PRX33/PRX34-generated ROS production is involved in the orchestration of pattern-triggered immunity in tissue culture cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.