ArticleThe Journal of membrane biology1990
Electrophysiology of cultured human lens epithelial cells.
Article in The Journal of membrane biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 47 citations in OpenAlex.
- Optimization of mouse kidney digestion protocols for single-cell applications.Physiological genomics · 2024Article
- Citrobacter rodentium colitis evokes post-infectious hyperexcitability of mouse nociceptive colonic dorsal root ganglion neurons.The Journal of physiology · 2009Article
- The lens circulation.The Journal of membrane biology · 2007Review
- Potassium currents in cultured rabbit retinal pigment epithelial cells.The Journal of membrane biology · 1994Article
- Expression of a tetrodotoxin-sensitive Na+ current in cultured human retinal pigment epithelial cells.The Journal of physiology · 1994Article
- Characterization of an outward potassium current in canine jejunal circular smooth muscle and its activation by fenamates.The Journal of physiology · 1993Article
- Single-membrane and cell-to-cell permeability properties of dissociated embryonic chick lens cells.The Journal of membrane biology · 1992Article
- Sodium channels in ocular epithelia.Pflugers Archiv : European journal of physiology · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The lens epithelial K+ conductance plays a key role in maintaining the lens ionic steady state. The specific channels responsible for this conductance are unknown. We used cultured lens epithelia and patch-clamp technology to address this problem. Human lens epithelial explants were cultured and after 1-4 passages were dissociated and used in this study. The cells from which we measured had a mean diameter of 31 +/- 1 microns (SEM, n = 26). The resting voltage was -19 +/- 4 mV (SEM, n = 10) and the input resistance was 2.5 +/- 0.5 G omega (SEM, n = 17) at -60 mV. Two currents were prominent in whole-cell recordings. An outwardly rectifying current was seen in nearly every cell. The magnitude of this current was a function of K+ concentration and was blocked by 3 mM tetraethylammonium. The instantaneous current-voltage relationship was linear in symmetric K+, implying that the outward rectification was due to gating. The current showed complex activation and inactivation kinetics. The second current seen was a transient inward current. This current had kinetics very similar to the traditional Na+ current of excitable cells and was blocked by 0.1 microM tetrodotoxin. In single-channel recordings, a 150-pS K+ channel and a 35-pS nonselective cation channel were seen but neither account for the macroscopic currents measured.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.