ArticleBMC research notes2012
Evolution of the eukaryotic ARP2/3 activators of the WASP family: WASP, WAVE, WASH, and WHAMM, and the proposed new family members WAWH and WAML.
Article in BMC research notes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 71 citations in OpenAlex.
- WHAMM deficiency alters splenic B-cell populations, BCR signaling, and actin remodeling.Molecular medicine (Cambridge, Mass.) · 2026Article
- Discovery of an Atypical Arp2/3 Complex in Malaria Parasites Sheds New Light on Nuclear Actin.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Recent insights into the plant ARP2/3 complex.Protoplasma · 2026Review
- An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission.Nature microbiology · 2025Article
- Cell signaling facilitates apical constriction by basolaterally recruiting Arp2/3 via Rac and WAVE.The Journal of cell biology · 2025Article
- Role of WAVE3 as an actin binding protein in the pathology of triple negative breast cancer.Cytoskeleton (Hoboken, N.J.) · 2025Review
- Cell signaling facilitates apical constriction by basolaterally recruiting Arp2/3 via Rac and WAVE.bioRxiv : the preprint server for biology · 2024Article
- Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes distant metastasis in pancreatic ductal adenocarcinoma via activation of LOXL2.Oncology research · 2024Article
- ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants.Nature plants · 2023Article
- Branching out in different directions: Emerging cellular functions for the Arp2/3 complex and WASP-family actin nucleation factors.European journal of cell biology · 2023Article
- Article
- The actin networks of chytrid fungi reveal evolutionary loss of cytoskeletal complexity in the fungal kingdom.Current biology : CB · 2021Article
- Conserved actin machinery drives microtubule-independent motility and phagocytosis in Naegleria.The Journal of cell biology · 2020Article
- Developmental Modulation of Root Cell Wall Architecture Confers Resistance to an Oomycete Pathogen.Current biology : CB · 2020Article
- WHIMP links the actin nucleation machinery to Src-family kinase signaling during protrusion and motility.PLoS genetics · 2020Article
- Article
- The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion.Molecular biology and evolution · 2019Article
- Investigation into Early Steps of Actin Recognition by the Intrinsically Disordered N-WASP Domain V.International journal of molecular sciences · 2019Article
- Comparative genomics reveals the origin of fungal hyphae and multicellularity.Nature communications · 2019Article
- Structural Characterization of N-WASP Domain V Using MD Simulations with NMR and SAXS Data.Biophysical journal · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWASP family proteins stimulate the actin-nucleating activity of the ARP2/3 complex. They include members of the well-known WASP and WAVE/Scar proteins, and the recently identified WASH and WHAMM proteins. WASP family proteins contain family specific N-terminal domains followed by proline-rich regions and C-terminal VCA domains that harbour the ARP2/3-activating regions.
resultsTo reveal the evolution of ARP2/3 activation by WASP family proteins we performed a "holistic" analysis by manually assembling and annotating all homologs in most of the eukaryotic genomes available. We have identified two new families: the WAML proteins (WASP and MIM like), which combine the membrane-deforming and actin bundling functions of the IMD domains with the ARP2/3-activating VCA regions, and the WAWH protein (WASP without WH1 domain) that have been identified in amoebae, Apusozoa, and the anole lizard. Surprisingly, with one exception we did not identify any alternative splice forms for WASP family proteins, which is in strong contrast to other actin-binding proteins like Ena/VASP, MIM, or NHS proteins that share domains with WASP proteins.
conclusionsOur analysis showed that the last common ancestor of the eukaryotes must have contained a homolog of WASP, WAVE, and WASH. Specific families have subsequently been lost in many taxa like the WASPs in plants, algae, Stramenopiles, and Euglenozoa, and the WASH proteins in fungi. The WHAMM proteins are metazoa specific and have most probably been invented by the Eumetazoa. The diversity of WASP family proteins has strongly been increased by many species- and taxon-specific gene duplications and multimerisations. All data is freely accessible via http://www.cymobase.org.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.