ArticleAnnals of biomedical engineering2012
Multi-scale structural and tensile mechanical response of annulus fibrosus to osmotic loading.
Article in Annals of biomedical engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- Experimental Assessment of Iris Water Content and Sex Differences in Mature Rabbits.bioRxiv : the preprint server for biology · 2026Article
- Structural Motifs in Soft Fibrous Tissues: Revealing Structure-Mechanics Relationships in Deformation and Tear Resistance for Biomimetic Material Design.Advanced healthcare materials · 2025Review
- Why are soft collagenous tissues so tough?Science advances · 2025Article
- Current Therapeutic Strategies of Intervertebral Disc Regenerative Medicine.Molecular diagnosis & therapy · 2024Review
- Article
- Potential molecular targets and drugs for basement membranes-related intervertebral disk degeneration through bioinformatics analysis and molecular docking.BMC musculoskeletal disorders · 2023Article
- Volume Loss and Recovery in Bovine Knee Meniscus Loaded in Circumferential Tension.Journal of biomechanical engineering · 2023Article
- Improving Mechanical Properties of Tendon Allograft through Rehydration Strategies: An In Vitro Study.Bioengineering (Basel, Switzerland) · 2023Article
- Pericellular heparan sulfate proteoglycans: Role in regulating the biosynthetic response of nucleus pulposus cells to osmotic loading.JOR spine · 2022Article
- A comprehensive tool box for large animal studies of intervertebral disc degeneration.JOR spine · 2021Article
- Evaluation of transverse poroelastic mechanics of tendon using osmotic loading and biphasic mixture finite element modeling.Journal of biomechanics · 2020Article
- Multiscale composite model of fiber-reinforced tissues with direct representation of sub-tissue properties.Biomechanics and modeling in mechanobiology · 2020Article
- Helical fibrillar microstructure of tendon using serial block-face scanning electron microscopy and a mechanical model for interfibrillar load transfer.Journal of the Royal Society, Interface · 2019Article
- Decellularization and characterization of a whole intervertebral disk xenograft scaffold.Journal of biomedical materials research. Part A · 2018Article
- Exposure to buffer solution alters tendon hydration and mechanics.Journal of biomechanics · 2017Article
- Region-media coupling in characterization and modelling of the disc annulus single lamella swelling.Medical & biological engineering & computing · 2017Article
- Tissue Engineering of the Intervertebral Disc's Annulus Fibrosus: A Scaffold-Based Review Study.Tissue engineering and regenerative medicine · 2017Review
- Novel human intervertebral disc strain template to quantify regional three-dimensional strains in a population and compare to internal strains predicted by a finite element model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Article
- Targeting the annulus fibrosus of the intervertebral disc: Col1a2-Cre(ER)T mice show specific activity of Cre recombinase in the outer annulus fibrosus.Journal of cell communication and signaling · 2016Article
- Human cartilage endplate permeability varies with degeneration and intervertebral disc site.Journal of biomechanics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
This study investigates differential multi-scale structure and function relationships of the outer and inner annulus fibrosus (AF) to osmotic swelling in different buffer solutions by quantifying tensile mechanics, glycoasamino-glycan(GAG) content, water content and tissue swelling, and collagen fibril ultrastructure. In the outer AF, the tensile modulus decreased by over 70% with 0.15 M PBS treatment but was unchanged with 2 M PBS treatment. Moreover, the modulus loss following 0.15 M PBS treatment was reversed when followed by 2 M PBS treatment, potentially from increased interfibrillar and interlamellar shearing associated with fibril swelling. In contrast, the inner AF tensile modulus was unchanged by 0.15 M PBS treatment and increased following 2 M treatment. Transmission electron microscopy revealed that the mean collagen fibril diameters of the untreated outer and inner AF were 87.8 ± 27.9 and 71.0 ± 26.9 nm, respectively. In the outer AF, collagen fibril swelling was observed with both 0.15 M and 2 M PBS treatments, but inherently low GAG content remained unchanged. In the inner AF, 2 M PBS treatment caused fibril swelling and GAG loss, suggesting that GAG plays a role in maintaining the structure of collagen fibrils leading to modulation of the native tissue mechanical properties. These results demonstrate important regional variations in structure and composition, and their influence on the heterogeneous mechanics of the AF. Moreover, because the composition and structure is altered as a consequence of progressive disk degeneration, quantification of these interactions is critical for study of the AF pathogenesis of degeneration and tissue engineering
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