ArticleJournal of proteome research2012
Regulation of lipid metabolism by Dicer revealed through SILAC mice.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- MicroRNAs as Epigenetic Regulators of Obesity.Advances in experimental medicine and biology · 2024Review
- Evolving Diagnostic and Treatment Strategies for Pediatric CNS Tumors: The Impact of Lipid Metabolism.Biomedicines · 2023Review
- Article
- MicroRNA regulation of cholesterol metabolism.Annals of the New York Academy of Sciences · 2021Review
- Intestinal miRNAs regulated in response to dietary lipids.Scientific reports · 2020Article
- Intestinal Lipid Metabolism Genes Regulated by miRNAs.Frontiers in genetics · 2020Article
- Function of Dicer with regard to Energy Homeostasis Regulation, Structural Modification, and Cellular Distribution.International journal of endocrinology · 2020Review
- Article
- The impact of lipids, lipid oxidation, and inflammation on AMD, and the potential role of miRNAs on lipid metabolism in the RPE.Experimental eye research · 2019Review
- Interplay of mitochondrial metabolism and microRNAs.Cellular and molecular life sciences : CMLS · 2017Review
- Ablation of Dicer leads to widespread perturbation of signaling pathways.Biochemical and biophysical research communications · 2015Article
- Adult-specific functions of animal microRNAs.Nature reviews. Genetics · 2013Review
- Identification of disease specific pathways using in vivo SILAC proteomics in dystrophin deficient mdx mouse.Molecular & cellular proteomics : MCP · 2013Article
- Proteomics for understanding miRNA biology.Proteomics · 2013Review
- The RNase III enzyme DROSHA is essential for microRNA production and spermatogenesis.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
Dicer is a ribonuclease whose major role is to generate mature microRNAs, although additional functions have been proposed. Deletion of Dicer leads to embryonic lethality in mice. To study the role of Dicer in adults, we generated mice in which administration of tamoxifen induces deletion of Dicer. Surprisingly, disruption of Dicer in adult mice induced lipid accumulation in the small intestine. To dissect the underlying mechanisms, we carried out miRNA, mRNA, and proteomic profiling of the small intestine. The proteomic analysis was done using mice metabolically labeled with heavy lysine (SILAC mice) for an in vivo readout. We identified 646 proteins, of which 80 were up-regulated >2-fold and 75 were down-regulated. Consistent with the accumulation of lipids, Dicer disruption caused a marked decrease of microsomal triglyceride transfer protein, long-chain fatty acyl-CoA ligase 5, fatty acid binding protein, and very-long-chain fatty acyl-CoA dehydrogenase, among others. We validated these results using multiple reaction monitoring (MRM) experiments by targeting proteotypic peptides. Our data reveal a previously unappreciated role of Dicer in lipid metabolism. These studies demonstrate that a systems biology approach by integrating mouse models, metabolic labeling, gene expression profiling, and quantitative proteomics can be a powerful tool for understanding complex biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.