ArticleNucleic acids research2012
Transgene regulation in plants by alternative splicing of a suicide exon.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Robust gene expression control in human cells with a novel universal TetR aptamer splicing module.Nucleic acids research · 2019Article
- A small, portable RNA device for the control of exon skipping in mammalian cells.Nucleic acids research · 2018Article
- Genome-wide cataloging and analysis of alternatively spliced genes in cereal crops.BMC genomics · 2015Article
- Tight regulation of plant immune responses by combining promoter and suicide exon elements.Nucleic acids research · 2015Article
- Transient gene expression in tobacco using Gibson assembly and the Gene Gun.Journal of visualized experiments : JoVE · 2014Article
- Complexity of the alternative splicing landscape in plants.The Plant cell · 2013Review
- Genome-wide landscape of alternative splicing events in Brachypodium distachyon.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compared to transcriptional activation, other mechanisms of gene regulation have not been widely exploited for the control of transgenes. One barrier to the general use and application of alternative splicing is that splicing-regulated transgenes have not been shown to be reliably and simply designed. Here, we demonstrate that a cassette bearing a suicide exon can be inserted into a variety of open reading frames (ORFs), generating transgenes whose expression is activated by exon skipping in response to a specific protein inducer. The surprisingly minimal sequence requirements for the maintenance of splicing fidelity and regulation indicate that this splicing cassette can be used to regulate any ORF containing one of the amino acids Glu, Gln or Lys. Furthermore, a single copy of the splicing cassette was optimized by rational design to confer robust gene activation with no background expression in plants. Thus, conditional splicing has the potential to be generally useful for transgene regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.