ArticleThe Journal of physiology2012
Advanced in vitro approach to study neurovascular coupling mechanisms in the brain microcirculation.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 53 citations in OpenAlex.
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- Central neural circuits and their associated mechanisms of inter-organ crosstalk.Frontiers in cell and developmental biology · 2026Review
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- Dynamic Neuro-Glial-Vascular Responses in a Mouse Model of Vascular Cognitive Impairment.Neuroglia (Basel, Switzerland) · 2024Article
- Astrocytes sense glymphatic-level shear stress through the interaction of sphingosine-1-phosphate with Piezo1.iScience · 2024Article
- Mechanobiological insight into brain diseases based on mechanosensitive channels: Common mechanisms and clinical potential.CNS neuroscience & therapeutics · 2024Review
- Intraluminal pressure elevates intracellular calcium and contracts CNS pericytes: Role of voltage-dependent calcium channels.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Two decades of astrocytes in neurovascular coupling.Frontiers in network physiology · 2023Review
- Three potential neurovascular pathways driving the benefits of mindfulness meditation for older adults.Frontiers in aging neuroscience · 2023Review
- Correction of Hypercortisolemia with an Improved Cognitive Function and Muscle Mass after Transsphenoidal Surgery in an Older Patient with Cushing's Disease.Internal medicine (Tokyo, Japan) · 2022Article
- The role of nitric oxide in flow-induced and myogenic responses in 1A, 2A, and 3A branches of the porcine middle cerebral artery.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- Advances in Hydrogel-Based Microfluidic Blood-Brain-Barrier Models in Oncology Research.Pharmaceutics · 2022Review
- Article
- Effects of choline containing phospholipids on the neurovascular unit: A review.Frontiers in cellular neuroscience · 2022Review
- Advances in the Role of Endothelial Cells in Cerebral Small Vessel Disease.Frontiers in neurology · 2022Review
- Guidelines for the measurement of vascular function and structure in isolated arteries and veins.American journal of physiology. Heart and circulatory physiology · 2021Review
- Decreased parenchymal arteriolar tone uncouples vessel-to-neuronal communication in a mouse model of vascular cognitive impairment.GeroScience · 2021Article
- Regional Haemodynamic and Metabolic Coupling in Infants.Frontiers in human neuroscience · 2021Article
- Imaging Pericytes and the Regulation of Cerebral Blood Flow.Methods in molecular biology (Clifton, N.J.) · 2021Article
- In Vitro and In Vivo Study of the Short-Term Vasomotor Response during Epileptic Seizures.Brain sciences · 2020Article
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2 authors at 1 institution in 1 country.
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Abstract
An understanding of the signalling events underlying neurovascular coupling mechanisms in the brain is a crucial step in the development of novel therapeutic approaches for the treatment of cerebrovascular-associated disorders. In this study we present an enhanced in vitro brain slice preparation from male Wistar rat cortical slices that incorporates haemodynamic variables (flow and pressure) into parenchymal arterioles resulting in the development of myogenic tone (28% from maximum dilatation). Moreover, we characterized flow-induced vascular responses, resulting in various degrees of vasoconstrictions and the response to 10 mM K(+) or astrocytic activation with the mGluR agonist, t-ACPD (100 μM), resulting in vasodilatations of 33.6±4.7% and 38.6±4.6%, respectively. Using fluorescence recovery, we determined perfusate velocity to calculate diameter changes under different experimental pH conditions. Using this approach, we demonstrate no significant differences between diameter changes measured using videomicroscopy or predicted from the velocity values obtained using fluorescence recovery after photobleaching. The model is further validated by demonstrating our ability to cannulate arterioles in two brain regions (cortex and supraoptic nucleus of the hypothalamus). Altogether, we believe this is the first study demonstrating successful cannulation and perfusion of parenchymal arterioles while monitoring/estimating luminal diameter and pressure under conditions where flow rates are controlled.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.