ReviewBioresource technology2012
Biocatalyst development by directed evolution.
Review in Bioresource technology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy.Molecular therapy. Oncology · 2026Article
- Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.Journal of the American Chemical Society · 2025Article
- Nitrile hydratase as a promising biocatalyst: recent advances and future prospects.Biotechnology letters · 2024Review
- Cell-Free Protein Expression in Polymer Materials.ACS synthetic biology · 2024Article
- Enzymatic Performance ofBioinformatics and biology insights · 2024Article
- Recombinant acetylxylan esterase of Halalkalibacterium halodurans NAH-Egypt: molecular and biochemical study.AMB Express · 2022Article
- Structure-Guided Engineering of a Family IV Cold-Adapted Esterase Expands Its Substrate Range.International journal of molecular sciences · 2022Article
- Droplet flow cytometry for single-cell analysis.RSC advances · 2021Review
- Article
- Application of a Dual Internally Quenched Fluorogenic Substrate in Screening for D-Arginine Specific Proteases.Frontiers in microbiology · 2019Article
- In-Silico Bioprospecting: Finding Better Enzymes.Molecular biotechnology · 2019Review
- Site-Directed Mutagenesis of a β-Glycoside Hydrolase fromMolecules (Basel, Switzerland) · 2018Article
- Review: Engineering of thermostable enzymes for industrial applications.APL bioengineering · 2018Review
- Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1-2 by Directed Evolution.Molecules (Basel, Switzerland) · 2017Article
- Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.Journal of the American Chemical Society · 2017Article
- Discovery of human-like L-asparaginases with potential clinical use by directed evolution.Scientific reports · 2017Article
- An Overview on the Enhancement of Enantioselectivity and Stability of Microbial Epoxide Hydrolases.Molecular biotechnology · 2017Review
- Directed evolution of a β-mannanase fromBiotechnology for biofuels · 2017Article
- Cellulolytic RoboLector - towards an automated high-throughput screening platform for recombinant cellulase expression.Journal of biological engineering · 2017Article
- The Need for Integrated Approaches in Metabolic Engineering.Cold Spring Harbor perspectives in biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial applications. Recent advances in mutant library creation and high-throughput screening have greatly facilitated the engineering of novel and improved biocatalysts. This review provides an update of the recent developments in the use of directed evolution to engineer biocatalysts for practical applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.