ArticleThe journal of trauma and acute care surgery2012
Electroporation-mediated in vivo gene delivery of the Na+/K+-ATPase pump reduced lung injury in a mouse model of lung contusion.
Article in The journal of trauma and acute care surgery, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Pulmonary gene delivery-Realities and possibilities.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Gene Therapy for Acute Respiratory Distress Syndrome.Frontiers in physiology · 2021Review
- TLR3 absence confers increased survival with improved macrophage activity against pneumonia.JCI insight · 2019Article
- Direct pulmonary delivery of solubilized curcumin reduces severity of lethal pneumonia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Electroporation-mediated delivery of FER gene enhances innate immune response and improves survival in a murine model of pneumonia.Gene therapy · 2018Article
- [The Use Of Pulmonary Gene Therapy In The Treatment Of Experimental Models Of Pneumonia And Septicemia].Gaceta medica de Caracas · 2018Article
- Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.Gene therapy · 2016Article
- A pliable electroporation patch (ep-Patch) for efficient delivery of nucleic acid molecules into animal tissues with irregular surface shapes.Scientific reports · 2015Article
- Electroporation-mediated gene delivery.Advances in genetics · 2015Article
- Electroporation-mediated gene delivery of Na+,K+ -ATPase, and ENaC subunits to the lung attenuates acute respiratory distress syndrome in a two-hit porcine model.Shock (Augusta, Ga.) · 2015Article
- Activation of hypoxia-inducible factor-1α in type 2 alveolar epithelial cell is a major driver of acute inflammation following lung contusion.Critical care medicine · 2014Article
- Alveolar macrophage depletion increases the severity of acute inflammation following nonlethal unilateral lung contusion in mice.The journal of trauma and acute care surgery · 2014Article
- Interleukin 10 overexpression alters survival in the setting of gram-negative pneumonia following lung contusion.Shock (Augusta, Ga.) · 2014Article
- Electroporation-mediated delivery of genes in rodent models of lung contusion.Methods in molecular biology (Clifton, N.J.) · 2014Article
- The protective role of MnTBAP in oxidant-mediated injury and inflammation in a rat model of lung contusion.Surgery · 2013Article
- A review of therapeutic prospects of non-viral gene therapy in the retinal pigment epithelium.Biomaterials · 2013Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLung contusion (LC) is an independent risk factor for acute respiratory distress syndrome. The final common pathway in ARDS involves accumulation of fluid in the alveoli. In this study, we demonstrate the application of a potential gene therapy approach by delivering the Na+/K+-ATPase pump subunits in a murine model of LC. We hypothesized that restoring the activity of the pump will result in removal of excess alveolar fluid and additionally reduce inflammation.
methodsUnder anesthesia, C57/BL6 mice were struck along the right posterior axillary line 1 cm above the costal margin with a cortical contusion impactor. Immediately afterward, 100 μg of plasmid DNA coding for the α,β of the Na+/K+-ATPase pump were instilled into the lungs (LC-electroporation-pump group). Contusion only (LC-only) and a sham saline instillation group after contusion were used as controls (LC-electroporation-sham). By using a BTX 830 electroporator, eight electrical pulses of 200 V/cm field strength were applied transthoracically. Mice were killed at 24 hours, 48 hours, and 72 hours after delivery. Bronchial alveolar lavage was recollected to measure albumin and cytokines by enzyme-linked immunosorbent assay. Pulmonary compliance was measured, and lungs were subject to histopathologic analysis.
resultsAfter the electroporation and delivery of genes coding for the α,β subunits of the Na+/K+-ATPase pump, there was a significant mitigation of acute lung injury as evidenced by reduction in bronchial alveolar lavage levels of albumin, improved pressure volume curves, and reduced inflammation seen on histology.
conclusionElectroporation-mediated gene transfer of the subunits of the Na+/K+-ATPase pump enhanced recovery from acute inflammatory lung injury after LC.
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