ArticleJournal of translational medicine2012
Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay.
Article in Journal of translational medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.
- The Role of Chronic Inflammation in Polycystic Ovarian Syndrome-A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2021Pooled it
- Quantum Dot-Based Fluorescence-Linked Immunosorbent Assays for the Detection of Biomarkers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Fabrication of GQD-Electrodeposited Screen-Printed Carbon Electrodes for the Detection of the CRP Biomarker.ACS omega · 2021Article
- Colorimetric cellulose-based test-strip for rapid detection of amyloid β-42.Mikrochimica acta · 2021Article
- Functionalized ultra-fine bimetallic PtRu alloy nanoparticle with high peroxidase-mimicking activity for rapid and sensitive colorimetric quantification of C-reactive protein.Mikrochimica acta · 2021Article
- Article
- Sensing with photoluminescent semiconductor quantum dots.Methods and applications in fluorescence · 2019Article
- Label-Free Electrochemical Immunoassay for C-Reactive Protein.Biosensors · 2018Article
- Synthesis of Reabsorption-Suppressed Type-II/Type-I ZnSe/CdS/ZnS Core/Shell Quantum Dots and Their Application for Immunosorbent Assay.Nanoscale research letters · 2017Article
- Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay.Journal of nanobiotechnology · 2017Article
- Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA).Beilstein journal of nanotechnology · 2017Article
- Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay.International journal of nanomedicine · 2015Article
- Quantum dots-based quantitative and in situ multiple imaging on ki67 and cytokeratin to improve ki67 assessment in breast cancer.PloS one · 2015Article
- Synthesis of water-soluble CdSe quantum dots with various fluorescent properties and their application in immunoassay for determination of C-reactive protein.Journal of fluorescence · 2014Article
- Clinical Significance of Cys-C and hs-CRP in Coronary Heart Disease Patients Undergoing Percutaneous Coronary Intervention.Brazilian journal of cardiovascular surgeryArticle
- Nanobiosensors in diagnostics.NanobiomedicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh-sensitivity C-reactive protein (hs-CRP) assay is of great clinical importance in predicting risks associated with coronary heart disease. Existing hs-CRP assays either require complex operation or have low throughput and cannot be routinely implemented in rural settings due to limited laboratory resources.
methodsWe developed a novel hs-CRP assay capable of simultaneously quantifying over 90 clinical samples by using quantum dots-labeled immunoassay within a standard 96-well microplate. The specificity of the assay was enhanced by adopting two monoclonal antibodies (mAbs) that target distinct hs-CRP epitopes, serving as the coating antibody and the detection antibody, respectively. In the presence of hs-CRP antigen, the fluorescence intensity of the mAb-Ag-mAb sandwich complex captured on the microplate can be read out using a microplate reader.
resultsThe proposed hs-CRP assay provides a wide analytical range of 0.001-100 mg/L with a detection limit of 0.06 (0.19) μg/L within 1.5 h. The accuracy of the proposed assay has been confirmed for low coefficient of variations (CVs), 2.27% (intra-assay) and 8.52% (inter-assay), together with recoveries of 96.7-104.2%. Bland-Altman plots of 104 clinical samples exhibited good consistency among the proposed assay, commercial high-sensitivity ELISA, and nephelometry, indicating the prospects of the newly developed hs-CRP assay as an alternative to existing hs-CRP assays.
conclusionThe developed assay meets the needs of the rapid, sensitive and high-throughput determination of hs-CRP levels within a short time using minimal resources. In addition, the developed assay can also be used to detect and quantify other diagnostic biomarkers by immobilizing specific monoclonal antibodies.
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