ArticleMolecular plant pathology2012
Melon RNA interference (RNAi) lines silenced for Cm-eIF4E show broad virus resistance.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 97 citations in OpenAlex.
- Decoding MicroRNA-Guided Antiviral Defense in Cucurbitaceae: Regulatory Networks, RNA Silencing Cross-Talk, and Emerging Strategies for Crop Resilience.International journal of molecular sciences · 2026Review
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Noncoding RNAs as tools for advancing translational biology in plants.The Plant cell · 2025Review
- Insights into the early transcriptomic response against watermelon mosaic virus in melon.BMC plant biology · 2024Article
- Allele mining of eukaryotic translation initiation factor genes in Prunus for the identification of new sources of resistance to sharka.Scientific reports · 2023Article
- Mutagenesis of the melon Prv gene by CRISPR/Cas9 breaks papaya ringspot virus resistance and generates an autoimmune allele with constitutive defense responses.Journal of experimental botany · 2023Article
- CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (International journal of molecular sciences · 2023Article
- Integrated Molecular and Bioinformatics Approaches for Disease-Related Genes in Plants.Plants (Basel, Switzerland) · 2023Review
- Plant eIF4E isoforms as factors of susceptibility and resistance to potyviruses.Frontiers in plant science · 2023Review
- Candidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sources.Frontiers in plant science · 2023Article
- Effect of Gene Silencing of Translation Initiation Factors eIF(iso)4G and eIF(iso)4E on Sour Cherry Rootstock Resistance to Sharka Disease.International journal of molecular sciences · 2022Article
- Different RNA Elements Control Viral Protein Synthesis in Polerovirus Isolates Evolved in Separate Geographical Regions.International journal of molecular sciences · 2022Article
- Editing melon eIF4E associates with virus resistance and male sterility.Plant biotechnology journal · 2022Article
- Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco.Scientific reports · 2022Article
- Small RNAs Participate in Plant-Virus Interaction and Their Application in Plant Viral Defense.International journal of molecular sciences · 2022Review
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions withThe plant pathology journal · 2021Article
- Current Advancements and Limitations of Gene Editing in Orphan Crops.Frontiers in plant science · 2021Review
- Construction of a high-density genetic linkage map and identification of gene controlling resistance to cucumber mosaic virus in Luffa cylindrica (L.) Roem. based on specific length amplified fragment sequencing.Molecular biology reports · 2020Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient and sustainable control of plant viruses may be achieved using genetically resistant crop varieties, although resistance genes are not always available for each pathogen; in this regard, the identification of new genes that are able to confer broad-spectrum and durable resistance is highly desirable. Recently, the cloning and characterization of recessive resistance genes from different plant species has pointed towards eukaryotic translation initiation factors (eIF) of the 4E family as factors required for the multiplication of many different viruses. Thus, we hypothesized that eIF4E may control the susceptibility of melon (Cucumis melo L.) to a broad range of viruses. To test this hypothesis, Cm-eIF4E knockdown melon plants were generated by the transformation of explants with a construct that was designed to induce the silencing of this gene, and the plants from T2 generations were genetically and phenotypically characterized. In transformed plants, Cm-eIF4E was specifically silenced, as identified by the decreased accumulation of Cm-eIF4E mRNA and the appearance of small interfering RNAs derived from the transgene, whereas the Cm-eIF(iso)4E mRNA levels remained unaffected. We challenged these transgenic melon plants with eight agronomically important melon-infecting viruses, and identified that they were resistant to Cucumber vein yellowing virus (CVYV), Melon necrotic spot virus (MNSV), Moroccan watermelon mosaic virus (MWMV) and Zucchini yellow mosaic virus (ZYMV), indicating that Cm-eIF4E controls melon susceptibility to these four viruses. Therefore, Cm-eIF4E is an efficient target for the identification of new resistance alleles able to confer broad-spectrum virus resistance in melon.
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