ArticleProceedings of the National Academy of Sciences of the United States of America2012
Leghemoglobin green derivatives with nitrated hemes evidence production of highly reactive nitrogen species during aging of legume nodules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 77 citations in OpenAlex.
- Maintaining sulfur supply to the symbiosome delays nodule senescence in soybean.Nature communications · 2025Article
- GmNAC039 and GmNAC018 activate the expression of cysteine protease genes to promote soybean nodule senescence.The Plant cell · 2023Article
- Signaling by reactive molecules and antioxidants in legume nodules.The New phytologist · 2022Review
- Multimodal Spectroscopic Imaging of Pea Root Nodules to Assess the Nitrogen Fixation in the Presence of Biofertilizer Based on Nod-Factors.International journal of molecular sciences · 2021Article
- Molecular responses of legumes to abiotic stress: post-translational modifications of proteins and redox signaling.Journal of experimental botany · 2021Review
- Characteristics and Research Progress of Legume Nodule Senescence.Plants (Basel, Switzerland) · 2021Review
- Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus.Microbes and environments · 2021Article
- Medicago truncatula Phytoglobin 1.1 controls symbiotic nodulation and nitrogen fixation via the regulation of nitric oxide concentration.The New phytologist · 2020Article
- Excess nitrate induces nodule greening and reduces transcript and protein expression levels of soybean leghaemoglobins.Annals of botany · 2020Article
- Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis inAntioxidants (Basel, Switzerland) · 2020Article
- Plant Nitrate Reductases Regulate Nitric Oxide Production and Nitrogen-Fixing Metabolism During theFrontiers in plant science · 2020Article
- The Role of Nitric Oxide in Nitrogen Fixation by Legumes.Frontiers in plant science · 2020Review
- Transcription Factor bHLH2 RepressesPlant physiology · 2019Article
- The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor.Redox biology · 2018Article
- Protein Carbonylation and Glycation in Legume Nodules.Plant physiology · 2018Article
- Nodulation and Delayed Nodule Senescence: Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen.Current microbiology · 2018Article
- Redefining nitric oxide production in legume nodules through complementary insights from electron paramagnetic resonance spectroscopy and specific fluorescent probes.Journal of experimental botany · 2018Article
- Modulation of the flavin-protein interactions in NADH peroxidase and mercuric ion reductase: a resonance Raman study.European biophysics journal : EBJ · 2018Article
- MtCAS31 Aids Symbiotic Nitrogen Fixation by Protecting the Leghemoglobin MtLb120-1 Under Drought Stress inFrontiers in plant science · 2018Article
- Functions of Nitric Oxide (NO) in Roots during Development and under Adverse Stress Conditions.Plants (Basel, Switzerland) · 2015Review
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Authors and funding
9 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Globins constitute a superfamily of proteins widespread in all kingdoms of life, where they fulfill multiple functions, such as efficient O(2) transport and modulation of nitric oxide bioactivity. In plants, the most abundant Hbs are the symbiotic leghemoglobins (Lbs) that scavenge O(2) and facilitate its diffusion to the N(2)-fixing bacteroids in nodules. The biosynthesis of Lbs during nodule formation has been studied in detail, whereas little is known about the green derivatives of Lbs generated during nodule senescence. Here we characterize modified forms of Lbs, termed Lba(m), Lbc(m), and Lbd(m), of soybean nodules. These green Lbs have identical globins to the parent red Lbs but their hemes are nitrated. By combining UV-visible, MS, NMR, and resonance Raman spectroscopies with reconstitution experiments of the apoprotein with protoheme or mesoheme, we show that the nitro group is on the 4-vinyl. In vitro nitration of Lba with excess nitrite produced several isomers of nitrated heme, one of which is identical to those found in vivo. The use of antioxidants, metal chelators, and heme ligands reveals that nitration is contingent upon the binding of nitrite to heme Fe, and that the reactive nitrogen species involved derives from nitrous acid and is most probably the nitronium cation. The identification of these green Lbs provides conclusive evidence that highly oxidizing and nitrating species are produced in nodules leading to nitrosative stress. These findings are consistent with a previous report showing that the modified Lbs are more abundant in senescing nodules and have aberrant O(2) binding.
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