ArticleProceedings of the National Academy of Sciences of the United States of America2012
pH-controlled delivery of luminescent europium coated nanoparticles into platelets.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Luminescent Lanthanides in Biorelated Applications: From Molecules to Nanoparticles and Diagnostic Probes to Therapeutics.Chemical reviews · 2025Review
- Aiming the magic bullet: targeted delivery of imaging and therapeutic agents to solid tumors by pHLIP peptides.Frontiers in pharmacology · 2024Review
- Cell-based carrier for targeted hitchhiking delivery.Drug delivery and translational research · 2022Review
- Rare earth smart nanomaterials for bone tissue engineering and implantology: Advances, challenges, and prospects.Bioengineering & translational medicine · 2022Review
- Roles of key residues and lipid dynamics reveal pHLIP-membrane interactions at intermediate pH.Biophysical journal · 2021Article
- Safety Evaluation of Nanotechnology Products.Pharmaceutics · 2021Review
- Mesoporous Silica Nanoparticles: Properties and Strategies for Enhancing Clinical Effect.Pharmaceutics · 2021Review
- Article
- Exploiting BBB disruption for the delivery of nanocarriers to the diseased CNS.Current opinion in biotechnology · 2019Review
- Assisted delivery of anti-tumour platinum drugs using DNA-coiling gold nanoparticles bearing lumophores and intercalators: towards a new generation of multimodal nanocarriers with enhanced action.Chemical science · 2019Article
- Nanophosphor-Based Contrast Agents for Spectral X-ray Imaging.Nanomaterials (Basel, Switzerland) · 2019Article
- Article
- Applications of pHLIP Technology for Cancer Imaging and Therapy.Trends in biotechnology · 2017Review
- Direct proof of spontaneous translocation of lipid-covered hydrophobic nanoparticles through a phospholipid bilayer.Science advances · 2016Article
- Hierarchical Targeting Strategy for Enhanced Tumor Tissue Accumulation/Retention and Cellular Internalization.Advanced materials (Deerfield Beach, Fla.) · 2016Article
- Article
- Article
- Recent advancements in erythrocytes, platelets, and albumin as delivery systems.OncoTargets and therapy · 2016Review
- The pH low insertion peptide pHLIP Variant 3 as a novel marker of acidic malignant lesions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Accessible Synthetic Probes for Staining Actin inside Platelets and Megakaryocytes by Employing Lifeact Peptide.Chembiochem : a European journal of chemical biology · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Water soluble, luminescent gold nanoparticles are delivered into human platelets via a rapid, pH-controlled mechanism using a pH low insertion peptide, pHLIP. The approach introduces cocoating of gold nanoparticles with a europium luminescent complex, EuL and the pHLIP peptide to give pHLIP•EuL•Au. The 13-nm diameter gold nanoparticles act as a scaffold for the attachment of both the luminescent probe and the peptide to target delivery. Their size allows delivery of approximately 640 lanthanide probes per nanoparticle to be internalized in human platelets, which are not susceptible to transfection or microinjection. The internalization of pHLIP•EuL•Au in platelets, which takes just minutes, was studied with a variety of imaging modalities including luminescence, confocal reflection, and transmission electron microscopy. The results show that pHLIP•EuL•Au only enters the platelets in low pH conditions, pH 6.5, mediated by the pHLIP translocation across the membrane, and not at pH 7.4. Luminescence microscopy images of the treated platelets show clearly the red luminescence signal from the europium probe and confocal reflection microscopy confirms the presence of the gold particles. Furthermore, transmission electron microscopy gives a detailed insight of the internalization and spatial localization of the gold nanoparticles in the platelets. Thus, we demonstrate the potential of the design to translocate multimodal nanoparticle probes into cells in a pH dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.