ArticleAmerican journal of physiology. Heart and circulatory physiology2012
Vena cava and aortic smooth muscle cells express transglutaminases 1 and 4 in addition to transglutaminase 2.
Article in American journal of physiology. Heart and circulatory physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Transglutaminase 1: Emerging Functions beyond Skin.International journal of molecular sciences · 2024Review
- Biochemical Characterisation of Human Transglutaminase 4.International journal of molecular sciences · 2021Article
- Probing tissue transglutaminase mediated vascular smooth muscle cell aging using a novel transamidation-deficient Tgm2-C277S mouse model.Cell death discovery · 2021Article
- Transglutaminases Are Active in Perivascular Adipose Tissue.International journal of molecular sciences · 2021Article
- Transglutaminase 2 as a Marker for Inflammation and Therapeutic Target in Sepsis.International journal of molecular sciences · 2021Review
- Article
- Serotonylation: Serotonin Signaling and Epigenetics.Frontiers in molecular neuroscience · 2019Review
- Long term gluten consumption in adults without celiac disease and risk of coronary heart disease: prospective cohort study.BMJ (Clinical research ed.) · 2017Article
- Involvement of transglutaminase 2 and voltage-gated potassium channels in cystamine vasodilatation in rat mesenteric small arteries.British journal of pharmacology · 2016Article
- Tissue Transglutaminase Mediated Tumor-Stroma Interaction Promotes Pancreatic Cancer Progression.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Decreased Mechanical Strength and Collagen Content in SPARC-Null Periodontal Ligament Is Reversed by Inhibition of Transglutaminase Activity.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
- Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls.American journal of physiology. Heart and circulatory physiology · 2015Article
- The obligatory role of the actin cytoskeleton on inward remodeling induced by dithiothreitol activation of endogenous transglutaminase in isolated arterioles.American journal of physiology. Heart and circulatory physiology · 2014Article
- Transglutaminse 2 and EGGL, the protein cross-link formed by transglutaminse 2, as therapeutic targets for disabilities of old age.Rejuvenation research · 2013Review
- Variations in both TG1 and TG2 isozyme-specific in situ activities and protein expressions during mouse embryonic development.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Transglutaminase inhibitors attenuate vascular calcification in a preclinical model.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Transglutaminase (TG) function facilitates several vascular processes and diseases. Although many of these TG-dependent vascular processes have been ascribed to the function of TG2, TG2 knockout mice have a mild vascular phenotype. We hypothesized that TGs besides TG2 exist and function in the vasculature. Biotin-pentylamide incorporation, a measure of general TG activity, was similar in wild-type and TG2 knockout mouse aortae, and the general TG inhibitor cystamine reduced biotin-pentylamine incorporation to a greater extent than the TG2-specific inhibitor Z-DON, indicating the presence of other functional TGs. Additionally, 5-hydroxytryptamine-induced aortic contraction, a TG-activity-dependent process, was decreased to a greater extent by general TG inhibitors vs. Z-DON (maximum contraction: cystamine = abolished, monodansylcadaverine = 28.6 ± 14.9%, and Z-DON = 60.2 ± 15.2% vehicle), providing evidence for the importance of TG2-independent activity in the vasculature. TG1, TG2, TG4, and Factor XIII (FXIII) mRNA in rat aortae and vena cavae was detected by RT-PCR. Western analysis detected TG1 and TG4, but not FXIII, in rat aortae and vena cavae and in TG2 knockout and wild-type mouse aortae. Immunostaining confirmed the presence of TG1, TG2, and TG4 in rat aortae and vena cavae, notably in smooth muscle cells; FXIII was absent. K5 and T26, FITC-labeled peptide substrates specific for active TG1 and TG2, respectively, were incorporated into rat aortae and vena cavae and wild-type, but not TG2 knockout, mouse aortae. These studies demonstrate that TG2-independent TG activity exists in the vasculature and that TG1 and TG4 are expressed in vascular tissues.
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