ArticleCurrent protocols in microbiology2012
Human cytomegalovirus: bacterial artificial chromosome (BAC) cloning and genetic manipulation.
Article in Current protocols in microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Human Cytomegalovirus UL4 is Required for Viral Reactivation via Cellular Reprogramming in Latently Infected CD34+ Progenitor Cells and Humanized Mice.bioRxiv : the preprint server for biology · 2026Article
- Cancer viroimmunotherapy platforms based on varicella-zoster virus and cytomegalovirus.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Review
- Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral libraries.Molecular therapy. Methods & clinical development · 2024Article
- Oncolytic varicella-zoster virus engineered with ORF8 deletion and armed with drug-controllable interleukin-12.Journal for immunotherapy of cancer · 2024Article
- Oncolytic α-herpesvirus and myeloid-tropic cytomegalovirus cooperatively enhance systemic antitumor responses.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus.International journal of molecular sciences · 2021Article
- OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Vaccine-Induced Skewing of T Cell Responses Protects Against Chikungunya Virus Disease.Frontiers in immunology · 2019Article
- The essential role of guinea pig cytomegalovirus (GPCMV) IE1 and IE2 homologs in viral replication and IE1-mediated ND10 targeting.Virology · 2017Article
- Human cytomegalovirus pUL79 is an elongation factor of RNA polymerase II for viral gene transcription.PLoS pathogens · 2014Article
- Murine cytomegalovirus protein pM92 is a conserved regulator of viral late gene expression.Journal of virology · 2014Article
- Impact of sequence variation in the UL128 locus on production of human cytomegalovirus in fibroblast and epithelial cells.Journal of virology · 2013Article
- Murine cytomegalovirus protein pM79 is a key regulator for viral late transcription.Journal of virology · 2013Article
- Cyclin A degradation by primate cytomegalovirus protein pUL21a counters its innate restriction of virus replication.PLoS pathogens · 2013Article
- Tale of a tegument transactivator: the past, present and future of human CMV pp71.Future virology · 2012Article
- Histone H3 lysine 4 methylation marks postreplicative human cytomegalovirus chromatin.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The understanding of human cytomegalovirus (HCMV) biology was long hindered by the inability to perform efficient viral genetic analysis. This hurdle was recently overcome when the genomes of multiple HCMV strains were cloned as infectious bacterial artificial chromosomes (BACs). The BAC system takes advantage of the single-copy F plasmid of E. coli that can stably carry large pieces of foreign DNA. In this system, a recombinant HCMV virus carrying a modified F plasmid is first generated in eukaryotic cells. Recombinant viral genomes are then isolated and recovered in E. coli as BAC clones. BAC-captured viral genomes can be manipulated using prokaryotic genetics, and recombinant virus can be reconstituted from BAC transfection in eukaryotic cells. The BAC reverse genetic system provides a reliable and efficient method to introduce genetic alterations into the viral genome in E.coli and subsequently analyze their effects on virus biology in eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.