ArticleApplied and environmental microbiology2012
Effect of soybean coumestrol on Bradyrhizobium japonicum nodulation ability, biofilm formation, and transcriptional profile.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Unusual Morphological Changes of a Novel Wrinkled Bacterium Isolated from the Rice Rhizosphere Under Nutrient Stress.Life (Basel, Switzerland) · 2025Article
- Whispers beneath the soil: soybean-microbe communication pathways in the rhizosphere.Frontiers in plant science · 2025Review
- Biosynthesis and metabolic engineering of isoflavonoids in model plants and crops: a review.Frontiers in plant science · 2024Review
- Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress.Life (Basel, Switzerland) · 2022Article
- Production of the plant hormone gibberellin by rhizobia increases host legume nodule size.The ISME journal · 2022Article
- Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15).BMC microbiology · 2022Article
- Alterations of Primary Metabolites in Root Exudates of Intercropped Cajanus cajan-Zea mays Modulate the Adaptation and Proteome of Ensifer (Sinorhizobium) fredii NGR234.Microbial ecology · 2022Article
- A Potential Role of Coumestrol in Soybean Leaf Senescence and Its Interaction With Phytohormones.Frontiers in plant science · 2021Article
- Can Leaves and Stems ofPharmaceuticals (Basel, Switzerland) · 2020Article
- Comprehensive RNA sequencing and co-expression network analysis to complete the biosynthetic pathway of coumestrol, a phytoestrogen.Scientific reports · 2019Article
- Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates.Scientific reports · 2017Article
- A toolbox of genes, proteins, metabolites and promoters for improving drought tolerance in soybean includes the metabolite coumestrol and stomatal development genes.BMC genomics · 2016Article
- Characterization of MocR, a GntR-like transcriptional regulator, in Bradyrhizobium japonicum: its impact on motility, biofilm formation, and soybean nodulation.Journal of microbiology (Seoul, Korea) · 2015Article
- Role of the extracytoplasmic function sigma factor CarQ in oxidative response of Bradyrhizobium japonicum.Journal of microbiology (Seoul, Korea) · 2015Article
- Effects of the Bradyrhizobium japonicum waaL (rfaL) Gene on Hydrophobicity, Motility, Stress Tolerance, and Symbiotic Relationship with Soybeans.International journal of molecular sciences · 2015Article
- Characterization of a Functional Role of the Bradyrhizobium japonicum Isocitrate Lyase in Desiccation Tolerance.International journal of molecular sciences · 2015Article
- Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria.Applied and environmental microbiology · 2015Article
- Understanding Water-Stress Responses in Soybean Using Hydroponics System-A Systems Biology Perspective.Frontiers in plant science · 2015Article
- Involvement of a novel genistein-inducible multidrug efflux pump of Bradyrhizobium japonicum early in the interaction with Glycine max (L.) Merr.Microbes and environments · 2013Article
- Effects of indole-3-acetic acid on the transcriptional activities and stress tolerance of Bradyrhizobium japonicum.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavonoids, secondary plant metabolites which mainly have a polyphenolic structure, play an important role in plant-microbe communications for nitrogen-fixing symbiosis. Among 10 polyphenolic compounds isolated from soybean roots in our previous study, coumestrol showed the highest antioxidant activity. In this study, its effect on the soybean nodulation was tested. The soybean symbiont Bradyrhizobium japonicum USDA110 pretreated with 20 μM coumestrol enhanced soybean nodulation by increasing the number of nodules 1.7-fold compared to the control. We also tested the effect of coumestrol on B. japonicum biofilm formation. At a concentration of 2 μM, coumestrol caused a higher degree of biofilm formation than two major soybean isoflavonoids, genistein and daidzein, although no biofilm formation was observed at a concentration of 20 μM each compound. A genome-wide transcriptional analysis was performed to obtain a comprehensive snapshot of the B. japonicum response to coumestrol. When the bacterium was incubated in 20 μM coumestrol for 24 h, a total of 371 genes (139 upregulated and 232 downregulated) were differentially expressed at a 2-fold cutoff with a q value of less than 5%. No common nod gene induction was found in the microarray data. However, quantitative reverse transcription-PCR (qRT-PCR) data showed that incubation for 12 h resulted in a moderate induction (ca. 2-fold) of nodD1 and nodABC, indicating that soybean coumestrol is a weak inducer of common nod genes. In addition, disruption of nfeD (bll4952) affected the soybean nodulation by an approximate 30% reduction in the average number of nodules.
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