ArticleThe EMBO journal2012
Coordination of multiple enzyme activities by a single PCNA in archaeal Okazaki fragment maturation.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Characterization of DnaB-DnaG Interaction in M. tuberculosis Using Small-Angle X-ray Scattering-Based Dissociation Assay.Chembiochem : a European journal of chemical biology · 2025Article
- Mechanistic investigation of human maturation of Okazaki fragments reveals slow kinetics.Nature communications · 2022Article
- Cryo-EM structures and biochemical insights into heterotrimeric PCNA regulation of DNA ligase.Structure (London, England : 1993) · 2022Article
- The combined DNA and RNA synthetic capabilities of archaeal DNA primase facilitate primer hand-off to the replicative DNA polymerase.Nature communications · 2022Article
- Structure of eukaryotic DNA polymerase δ bound to the PCNA clamp while encircling DNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- A hand-off of DNA between archaeal polymerases allows high-fidelity replication to resume at a discrete intermediate three bases past 8-oxoguanine.Nucleic acids research · 2020Article
- Gene cloning and characterization of Tk1281, a flap endonuclease 1 from Thermococcus kodakarensis.Folia microbiologica · 2020Article
- Direct visualization of DNA baton pass between replication factors bound to PCNA.Scientific reports · 2018Article
- Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster.Nature communications · 2017Article
- Characterization of a coupled DNA replication and translesion synthesis polymerase supraholoenzyme from archaea.Nucleic acids research · 2017Article
- Review
- Eukaryotic DNA Replication Fork.Annual review of biochemistry · 2017Review
- Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme.Nature communications · 2017Article
- Evolution of the archaeal and mammalian information processing systems: towards an archaeal model for human disease.Cellular and molecular life sciences : CMLS · 2017Review
- DNA Polymerases Divide the Labor of Genome Replication.Trends in cell biology · 2016Review
- Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer.Scientific reports · 2016Article
- Resolving individual steps of Okazaki-fragment maturation at a millisecond timescale.Nature structural & molecular biology · 2016Article
- Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chromosomal DNA replication requires one daughter strand-the lagging strand-to be synthesised as a series of discontinuous, RNA-primed Okazaki fragments, which must subsequently be matured into a single covalent DNA strand. Here, we describe the reconstitution of Okazaki fragment maturation in vitro using proteins derived from the archaeon Sulfolobus solfataricus. Six proteins are necessary and sufficient for coupled DNA synthesis, RNA primer removal and DNA ligation. PolB1, Fen1 and Lig1 provide the required catalytic activities, with coordination of their activities dependent upon the DNA sliding clamp, proliferating cell nuclear antigen (PCNA). S. solfataricus PCNA is a heterotrimer, with each subunit having a distinct specificity for binding PolB1, Fen1 or Lig1. Our data demonstrate that the most efficient coupling of activities occurs when a single PCNA ring organises PolB1, Fen1 and Lig1 into a complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.