ArticleNature methods2012
Systematic identification of gene annotation errors in the widely used yeast mutation collections.
Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Many paths to destruction: Family-specific turnover and stress responses for tRNA introns.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- MANY PATHS TO DESTRUCTION: FAMILY-SPECIFIC TURNOVER AND STRESS RESPONSES FOR TRNA INTRONS.bioRxiv : the preprint server for biology · 2025Article
- Systematic Characterization and Analysis of the Freeze-Thaw Tolerance Gene Set in the Budding Yeast,International journal of molecular sciences · 2025Article
- The transcriptional coordination of functional gene clusters is dependent on multiple chromatin remodelers in a haploid strain of the budding yeast,Frontiers in fungal biology · 2025Article
- Article
- Copy number variation alters local and global mutational tolerance.Genome research · 2023Article
- Global analysis of the yeast knockout phenome.Science advances · 2023Article
- Functional Clustering of Metabolically Related Genes Is Conserved acrossJournal of fungi (Basel, Switzerland) · 2023Review
- Transgene insertion into the plastid genome alters expression of adjacent native chloroplast genes at the transcriptional and translational levels.Plant biotechnology journal · 2023Article
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- Overexpression approaches to advance understanding of Candida albicans.Molecular microbiology · 2022Review
- A standard knockout procedure alters expression of adjacent loci at the translational level.Nucleic acids research · 2021Article
- Article
- A case of convergent-gene interference in the budding yeast knockout library causing chromosome instability.G3 (Bethesda, Md.) · 2021Article
- Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.Current genetics · 2020Article
- Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome inMicrobial cell (Graz, Austria) · 2020Article
- A Genome-Wide Screen for Genes Affecting Spontaneous Direct-Repeat Recombination inG3 (Bethesda, Md.) · 2020Article
- The kinase Isr1 negatively regulates hexosamine biosynthesis in S. cerevisiae.PLoS genetics · 2020Article
- Genomic Considerations for the Modification ofMicroorganisms · 2020Review
- Genomic clustering within functionally related gene families inComputational and structural biotechnology journal · 2020Review
1 more citing papers are in PubMed but not listed here.
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The baker's yeast mutation collections are extensively used genetic resources that are the basis for many genome-wide screens and new technologies. Anecdotal evidence has previously pointed to the putative existence of a neighboring gene effect (NGE) in these collections. NGE occurs when the phenotype of a strain carrying a particular perturbed gene is due to the lack of proper function of its adjacent gene. Here we performed a large-scale study of NGEs, presenting a network-based algorithm for detecting NGEs and validating software predictions using complementation experiments. We applied our approach to four datasets uncovering a similar magnitude of NGE in each (7-15%). These results have important consequences for systems biology, as the mutation collections are extensively used in almost every aspect of the field, from genetic network analysis to functional gene annotation.
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