ArticleThe Journal of experimental medicine1990
Characterization of a receptor for interleukin 5 on human eosinophils: variable expression and induction by granulocyte/macrophage colony-stimulating factor.
Article in The Journal of experimental medicine, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 105 citations in OpenAlex.
- CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production.Scientific reports · 2017Article
- Evaluation of peroxisome proliferator-activated receptor agonists on interleukin-5-induced eosinophil differentiation.Immunology · 2014Article
- Eosinophils induce airway smooth muscle cell proliferation.Journal of clinical immunology · 2013Article
- Up-regulation and activation of eosinophil integrins in blood and airway after segmental lung antigen challenge.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Expression and in vitro properties of guinea pig IL-5: comparison to human and murine orthologs.Mediators of inflammation · 2000Article
- Association of RACK1 and PKCbeta with the common beta-chain of the IL-5/IL-3/GM-CSF receptor.Oncogene · 1999Article
- Familial eosinophilia maps to the cytokine gene cluster on human chromosomal region 5q31-q33.American journal of human genetics · 1998Article
- Allergen-induced increases in IL-5 receptor alpha-subunit expression on bone marrow-derived CD34+ cells from asthmatic subjects. A novel marker of progenitor cell commitment towards eosinophilic differentiation.The Journal of clinical investigation · 1997Article
- Article
- Tissue eosinophilia induced by recombinant human interleukin-5 in the hamster cheek pouch membrane.Mediators of inflammation · 1995Article
- 1,1'-Ethylidenebis(tryptophan) (Peak E) induces functional activation of human eosinophils and interleukin 5 production from T lymphocytes: association of eosinophilia-myalgia syndrome with a L-tryptophan contaminant.Journal of clinical immunology · 1994Article
- Reconstitution of the functional receptors for murine and human interleukin 5.The Journal of experimental medicine · 1993Article
- Mature eosinophils stimulated to develop in human-cord blood mononuclear cell cultures supplemented with recombinant human interleukin-5. II. Vesicular transport of specific granule matrix peroxidase, a mechanism for effecting piecemeal degranulation.The American journal of pathology · 1992Article
- Molecular cloning and expression of the human interleukin 5 receptor.The Journal of experimental medicine · 1992Article
- The role of GM-CSF in infection.Infection · 1992Review
- Interleukin 5 messenger RNA expression by eosinophils in the intestinal mucosa of patients with coeliac disease.The Journal of experimental medicine · 1992Article
- Eosinophils: a review.Veterinary research communications · 1992Review
- Effects of IL-5, granulocyte/macrophage colony-stimulating factor (GM-CSF) and IL-3 on the survival of human blood eosinophils in vitro.Clinical and experimental immunology · 1991Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin 5 (IL-5) acts on eosinophil differentiation and activation, suggesting the existence of a membrane receptor for IL-5 on eosinophils. Here, we report that 125I-labeled recombinant human IL-5 bound, at 4 degrees C, to high affinity receptors on human eosinophils. The association constant was higher for hypodense eosinophils (1.93 x 10(9) M-1) than for normodense cells (0.39 x 10(9) M-1), with a closely related number of receptor sites per cell. No specific binding occurred on neutrophils. The specific binding of IL-5 was induced by overnight incubation at 37 degrees C of human eosinophils with granulocyte/macrophage (GM)-CSF. The levels of increase were significantly higher for normodense than for hypodense eosinophils, suggesting a previous in vivo activation of the later subpopulation by GM-CSF. IL-3 was ineffective by itself but synergistically enhanced the effect of GM-CSF. Specificity studies showed that the binding of 125I-labeled IL-5 was inhibited by IL-5, but not by other cytokines, on human eosinophils. These results show the existence of a specific binding site for IL-5 on human eosinophils with a variable affinity on eosinophil hypodense or normodense subpopulations, as previously reported for other membrane receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.