ArticleJournal of molecular biology2012
Isolation of an asymmetric RNA uncoating intermediate for a single-stranded RNA plant virus.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus.Science advances · 2025Article
- Review
- StructureMan: A Structure Manipulation Tool to Study Large Scale Biomolecular Interactions.Frontiers in molecular biosciences · 2020Article
- Visualization of Calcium Ion Loss from Rotavirus during Cell Entry.Journal of virology · 2018Article
- RNA virus evasion of nonsense-mediated decay.PLoS pathogens · 2018Article
- Biochemical and genetic functional dissection of the P38 viral suppressor of RNA silencing.RNA (New York, N.Y.) · 2017Article
- Evidence that Hsc70 Is Associated with Cucumber Necrosis Virus Particles and Plays a Role in Particle Disassembly.Journal of virology · 2017Article
- New Structural Insights into the Genome and Minor Capsid Proteins of BK Polyomavirus using Cryo-Electron Microscopy.Structure (London, England : 1993) · 2016Article
- Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2.Journal of molecular biology · 2016Article
- Uncoating Mechanism of Carnation Mottle Virus Revealed by Cryo-EM Single Particle Analysis.Scientific reports · 2015Article
- Cis- and trans-regulation of luteovirus gene expression by the 3' end of the viral genome.Virus research · 2015Review
- Calcium ions modulate the mechanics of tomato bushy stunt virus.Biophysical journal · 2015Article
- Asymmetric genome organization in an RNA virus revealed via graph-theoretical analysis of tomographic data.PLoS computational biology · 2015Article
- The Generation of Turnip Crinkle Virus-Like Particles in Plants by the Transient Expression of Wild-Type and Modified Forms of Its Coat Protein.Frontiers in plant science · 2015Article
- Sequence Conservation, Radial Distance and Packing Density in Spherical Viral Capsids.PloS one · 2015Article
- Pathways for virus assembly around nucleic acids.Journal of molecular biology · 2014Article
- Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant.Virology · 2014Article
- The asymmetric structure of an icosahedral virus bound to its receptor suggests a mechanism for genome release.Structure (London, England : 1993) · 2013Article
- A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence.RNA biology · 2013Article
- Sequence-specific, RNA-protein interactions overcome electrostatic barriers preventing assembly of satellite tobacco necrosis virus coat protein.Journal of molecular biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We have determined the three-dimensional structures of both native and expanded forms of turnip crinkle virus (TCV), using cryo-electron microscopy, which allows direct visualization of the encapsidated single-stranded RNA and coat protein (CP) N-terminal regions not seen in the high-resolution X-ray structure of the virion. The expanded form, which is a putative disassembly intermediate during infection, arises from a separation of the capsid-forming domains of the CP subunits. Capsid expansion leads to the formation of pores that could allow exit of the viral RNA. A subset of the CP N-terminal regions becomes proteolytically accessible in the expanded form, although the RNA remains inaccessible to nuclease. Sedimentation velocity assays suggest that the expanded state is metastable and that expansion is not fully reversible. Proteolytically cleaved CP subunits dissociate from the capsid, presumably leading to increased electrostatic repulsion within the viral RNA. Consistent with this idea, electron microscopy images show that proteolysis introduces asymmetry into the TCV capsid and allows initial extrusion of the genome from a defined site. The apparent formation of polysomes in wheat germ extracts suggests that subsequent uncoating is linked to translation. The implication is that the viral RNA and its capsid play multiple roles during primary infections, consistent with ribosome-mediated genome uncoating to avoid host antiviral activity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.