ArticleJournal of molecular biology2012
Cryo-EM structure of the archaeal 50S ribosomal subunit in complex with initiation factor 6 and implications for ribosome evolution.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 48 citations in OpenAlex.
- Structural basis of nucleotide-dependent conformational switching of a CBS-family protein on the archaeal 50S ribosome.Science China. Life sciences · 2026Article
- Cryo-EM study and in vivo chemical mapping of the Methanosarcina acetivorans ribosome and its dimerization via a repurposed enzyme and translation factor.The Journal of biological chemistry · 2025Article
- Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.Nature communications · 2025Article
- Assembly of the bacterial ribosome with circularly permuted rRNA.Nucleic acids research · 2024Article
- Expanded phylogeny of extremely halophilic archaea shows multiple independent adaptations to hypersaline environments.Nature microbiology · 2024Article
- Costs of ribosomal RNA stabilization affect ribosome composition at maximum growth rate.Communications biology · 2024Article
- In vivo structure probing of RNA inRNA (New York, N.Y.) · 2023Article
- Role of aIF5B in archaeal translation initiation.Nucleic acids research · 2022Article
- Non-essential ribosomal proteins in bacteria and archaea identified using COGs.Journal of bacteriology · 2021Article
- Coevolution of ribosomal RNA expansion segment 7L and assembly factor Noc2p specializes the ribosome biogenesis pathway between Saccharomyces cerevisiae and Candida albicans.Nucleic acids research · 2021Article
- Expansion segments in bacterial and archaeal 5S ribosomal RNAs.RNA (New York, N.Y.) · 2021Article
- The Archaeal Elongation Factor EF-2 Induces the Release of aIF6 From 50S Ribosomal Subunit.Frontiers in microbiology · 2021Article
- Cryo-electron microscopy visualization of a large insertion in the 5S ribosomal RNA of the extremely halophilic archaeon Halococcus morrhuae.FEBS open bio · 2020Article
- Structural Heterogeneities of the Ribosome: New Frontiers and Opportunities for Cryo-EM.Molecules (Basel, Switzerland) · 2020Review
- Selective Translation of Low Abundance and Upregulated Transcripts in Halobacterium salinarum.mSystems · 2020Article
- The 23S Ribosomal RNA FromFrontiers in microbiology · 2020Article
- Functional Interactions of Ribosomal Intersubunit Bridges inGenetics · 2019Article
- Revising the Structural Diversity of Ribosomal Proteins Across the Three Domains of Life.Molecular biology and evolution · 2018Article
- The Jigsaw Puzzle of mRNA Translation Initiation in Eukaryotes: A Decade of Structures Unraveling the Mechanics of the Process.Annual review of biophysics · 2018Article
- Evolution of the archaeal and mammalian information processing systems: towards an archaeal model for human disease.Cellular and molecular life sciences : CMLS · 2017Review
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7 authors at 3 institutions in 3 countries.
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Abstract
Translation of mRNA into proteins by the ribosome is universally conserved in all cellular life. The composition and complexity of the translation machinery differ markedly between the three domains of life. Organisms from the domain Archaea show an intermediate level of complexity, sharing several additional components of the translation machinery with eukaryotes that are absent in bacteria. One of these translation factors is initiation factor 6 (IF6), which associates with the large ribosomal subunit. We have reconstructed the 50S ribosomal subunit from the archaeon Methanothermobacter thermautotrophicus in complex with archaeal IF6 at 6.6 Å resolution using cryo-electron microscopy (EM). The structure provides detailed architectural insights into the 50S ribosomal subunit from a methanogenic archaeon through identification of the rRNA expansion segments and ribosomal proteins that are shared between this archaeal ribosome and eukaryotic ribosomes but are mostly absent in bacteria and in some archaeal lineages. Furthermore, the structure reveals that, in spite of highly divergent evolutionary trajectories of the ribosomal particle and the acquisition of novel functions of IF6 in eukaryotes, the molecular binding of IF6 on the ribosome is conserved between eukaryotes and archaea. The structure also provides a snapshot of the reductive evolution of the archaeal ribosome and offers new insights into the evolution of the translation system in archaea.
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