ArticleDNA repair2012
Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature.
Article in DNA repair, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Dynamics and thermal stability of the bypass polymerase, DinB homolog (Dbh).Frontiers in molecular biosciences · 2024Article
- Translesion synthesis of apurinic/apyrimidic site analogues by Y-family DNA polymerase Dbh fromActa biochimica et biophysica Sinica · 2022Article
- Enzymatic Switching Between Archaeal DNA Polymerases Facilitates Abasic Site Bypass.Frontiers in microbiology · 2021Article
- Heterotrimeric PCNA increases the activity and fidelity of Dbh, a Y-family translesion DNA polymerase prone to creating single-base deletion mutations.DNA repair · 2020Article
- Genetic Control of Oxidative Mutagenesis inJournal of bacteriology · 2020Article
- PolB1 Is Sufficient for DNA Replication and Repair Under Normal Growth Conditions in the Extremely Thermophilic CrenarchaeonFrontiers in microbiology · 2020Article
- A Unique B-Family DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota.Frontiers in microbiology · 2020Article
- Participation of UV-regulated Genes in the Response to Helix-distorting DNA Damage in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius.Microbes and environments · 2019Article
- How a Genetically Stable Extremophile Evolves: Modes of Genome Diversification in the Archaeon Sulfolobus acidocaldarius.Journal of bacteriology · 2017Article
- Article
- Archaeal replicative primases can perform translesion DNA synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Understanding DNA Repair in Hyperthermophilic Archaea: Persistent Gaps and Other Reasons to Focus on the Fork.Archaea (Vancouver, B.C.) · 2015Review
- Endogenous mutagenesis in recombinant sulfolobus plasmids.Journal of bacteriology · 2013Article
- Differential temperature-dependent multimeric assemblies of replication and repair polymerases on DNA increase processivity.Biochemistry · 2012Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The intrinsically thermostable Y-family DNA polymerases of Sulfolobus spp. have revealed detailed three-dimensional structure and catalytic mechanisms of trans-lesion DNA polymerases, yet their functions in maintaining their native genomes remain largely unexplored. To identify functions of the Y-family DNA polymerase Dbh in replicating the Sulfolobus genome under extreme conditions, we disrupted the dbh gene in Sulfolobus acidocaldarius and characterized the resulting mutant strains phenotypically. Disruption of dbh did not cause any obvious growth defect, sensitivity to any of several DNA-damaging agents, or change in overall rate of spontaneous mutation at a well-characterized target gene. Loss of dbh did, however, cause significant changes in the spectrum of spontaneous forward mutation in each of two orthologous target genes of different sequence. Relative to wild-type strains, dbh(-) constructs exhibited fewer frame-shift and other small insertion-deletion mutations, but exhibited more base-pair substitutions that converted G:C base pairs to T:A base pairs. These changes, which were confirmed to be statistically significant, indicate two distinct activities of the Dbh polymerase in Sulfolobus cells growing under nearly optimal culture conditions (78-80°C and pH 3). The first activity promotes slipped-strand events within simple repetitive motifs, such as mononucleotide runs or triplet repeats, and the second promotes insertion of C opposite a potentially miscoding form of G, thereby avoiding G:C to T:A transversions.
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