ArticleMalaria journal2012
Cytoadherence and virulence - the case of Plasmodium knowlesi malaria.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- IGFBP7 induces phenotypic shift from platelet-mediated autoagglutination to rosetting ofVirulence · 2026Article
- Aptamer-Based Biosensors for the Detection of Malaria.Biosensors · 2026Review
- SICA-mediated cytoadhesion of Plasmodium knowlesi-infected red blood cells to human umbilical vein endothelial cells.Scientific reports · 2022Article
- A member of the tryptophan-rich protein family is required for efficient sequestration of Plasmodium berghei schizonts.PLoS pathogens · 2022Article
- Ectopic Expression ofMicroorganisms · 2022Article
- Experimental Models to Study the Pathogenesis of Malaria-Associated Acute Respiratory Distress Syndrome.Frontiers in cellular and infection microbiology · 2022Review
- Article
- Surface area-to-volume ratio, not cellular viscoelasticity, is the major determinant of red blood cell traversal through small channels.Cellular microbiology · 2021Article
- Cytoadherence Properties ofFrontiers in microbiology · 2021Article
- Clinical management of Plasmodium knowlesi malaria.Advances in parasitology · 2021Review
- Knowlesi malaria: Human risk factors, clinical spectrum, and pathophysiology.Advances in parasitology · 2021Article
- An exported kinase family mediates species-specific erythrocyte remodelling and virulence in human malaria.Nature microbiology · 2020Article
- Renal detection of Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi in malaria associated acute kidney injury: a retrospective case-control study.BMC research notes · 2020Article
- Functional genomics of simian malaria parasites and host-parasite interactions.Briefings in functional genomics · 2019Review
- Tissue tropism in parasitic diseases.Open biology · 2019Review
- Multimodal analysis of Plasmodium knowlesi-infected erythrocytes reveals large invaginations, swelling of the host cell, and rheological defects.Cellular microbiology · 2019Article
- Case Report:The American journal of tropical medicine and hygiene · 2018Article
- Article
- Review
- The Duffy binding protein (PkDBPαII) of Plasmodium knowlesi from Peninsular Malaysia and Malaysian Borneo show different binding activity level to human erythrocytes.Malaria journal · 2017Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundCytoadherence of infected red blood cells to brain endothelium is causally implicated in malarial coma, one of the severe manifestations of falciparum malaria. Cytoadherence is mediated by specific binding of variant parasite antigens, expressed on the surface of infected erythrocytes, to endothelial receptors including, ICAM-1, VCAM and CD36. In fatal cases of severe falciparum malaria with coma, blood vessels in the brain are characteristically congested with infected erythrocytes. Brain sections from a fatal case of knowlesi malaria, but without coma, were similarly congested with infected erythrocytes. The objective of this study was to determine the binding phenotype of Plasmodium knowlesi infected human erythrocytes to recombinant human ICAM-1, VCAM and CD36.
methodsFive patients with PCR-confirmed P. knowlesi malaria were recruited into the study with consent between April and August 2010. Pre-treatment venous blood was washed and cultured ex vivo to increase the proportion of schizont-infected erythrocytes. Cultured blood was seeded into Petri dishes with triplicate areas coated with ICAM-1, VCAM and CD36. Following incubation at 37°C for one hour the dishes were washed and the number of infected erythrocytes bound/mm2 to PBS control areas and to recombinant human ICAM-1 VCAM and CD36 coated areas were recorded. Each assay was performed in duplicate. Assay performance was monitored with the Plasmodium falciparum clone HB3.
resultsBlood samples were cultured ex vivo for up to 14.5 h (mean 11.3 ± 1.9 h) to increase the relative proportion of mature trophozoite and schizont-infected red blood cells to at least 50% (mean 65.8 ± 17.51%). Three (60%) isolates bound significantly to ICAM-1 and VCAM, one (20%) isolate bound to VCAM and none of the five bound significantly to CD36.
conclusionsPlasmodium knowlesi infected erythrocytes from human subjects bind in a specific but variable manner to the inducible endothelial receptors ICAM-1 and VCAM. Binding to the constitutively-expressed endothelial receptor CD36 was not detected. Further work will be required to define the pathological consequences of these interactions.
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