ArticleAnalytical chemistry2012
Glycan analysis by reversible reaction to hydrazide beads and mass spectrometry.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Differential analysis of core-fucosylated glycoproteomics enabled by single-step truncation of N-glycans.Glycoconjugate journal · 2023Article
- High-Throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags.Journal of proteome research · 2023Article
- Resin and Magnetic Nanoparticle-Based Free Radical Probes for Glycan Capture, Isolation, and Structural Characterization.Analytical chemistry · 2019Article
- Efficient enrichment of glycopeptides by supramolecular nanoassemblies that use proximity-assisted covalent binding.The Analyst · 2019Article
- Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.Analytical chemistry · 2018Article
- N-glycan in cockroach allergen regulates human basophil function.Immunity, inflammation and disease · 2017Article
- High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization.Scientific reports · 2017Article
- Simultaneous quantification of N- and O-glycans using a solid-phase method.Nature protocols · 2017Article
- Discovery of Non-invasive Glycan Biomarkers for Detection and Surveillance of Gastric Cancer.Journal of Cancer · 2017Article
- Simultaneous analyses of N-linked and O-linked glycans of ovarian cancer cells using solid-phase chemoenzymatic method.Clinical proteomics · 2017Article
- Review
- Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides.Nature biotechnology · 2016Article
- QUANTITY: An Isobaric Tag for Quantitative Glycomics.Scientific reports · 2015Article
- Integrated glycoprotein immobilization method for glycopeptide and glycan analysis of cardiac hypertrophy.Analytical chemistry · 2015Article
- Glycomic analysis by glycoprotein immobilization for glycan extraction and liquid chromatography on microfluidic chip.Analytical chemistry · 2013Article
- Glycan analysis by isobaric aldehyde reactive tags and mass spectrometry.Analytical chemistry · 2013Article
- Glycomic analysis using glycoprotein immobilization for glycan extraction.Analytical chemistry · 2013Article
- Solid-phase glycan isolation for glycomics analysis.Proteomics. Clinical applications · 2012Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Investigation into glycoproteins and their associated glycans is the key to understanding the function of glycoproteins in biological pathways and disease development. Current methods for glycan analysis are generally based on multiple preparation processes to separate glycans from proteins and other molecules prior to analysis. During the multistep purification processes, glycans are continuously lost and the procedure increases the difficulty for accurate quantitative analysis of glycans. Here we describe the development of a novel technique, which uses hydrazide beads to capture glycans. It is based on the conjugation of glycans to hydrazide beads through the formation of reversible hydrazone, washing out unbound nonglycans, then releasing captured glycans by acids. The results showed that the glycans were able to be isolated from concatenate peptides by using hydrazide beads. This technique was also applied to the analysis of glycans from sera sample. The integrated capture-release on the solid-phase simplifies the procedure for glycan preparation from a complex mixture and can be a powerful tool for glycan analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.