ReviewBioanalysis2012
Replacing immunoassays with tryptic digestion-peptide immunoaffinity enrichment and LC-MS/MS.
Review in Bioanalysis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Design of experiments for the optimization of sample preparation for bottom-up targeted protein LC-MS/MS workflows.Bioanalysis · 2025Article
- Targeted Mass Spectrometry for Quantification of Receptor Tyrosine Kinase Signaling.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantitative analysis of therapeutic proteins in biological fluids: recent advancement in analytical techniques.Drug delivery · 2023Review
- Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing.ACS nano · 2022Article
- Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part I-Development.Annals of laboratory medicine · 2022Review
- Improved Sensitivity of Allergen Detection by Immunoaffinity LC-MS/MS Using Ovalbumin as a Case Study.Foods (Basel, Switzerland) · 2021Article
- A Mechanistic Site-Of-Action Model: A Tool for Informing Right Target, Right Compound, And Right Dose for Therapeutic Antagonistic Antibody Programs.Frontiers in bioinformatics · 2021Article
- A new mass spectrometry-based method for the quantification of histones in plasma from septic shock patients.Scientific reports · 2017Article
- Mass Spectrometry Applications for Toxicology.EJIFCC · 2016Article
- Immunocapture strategies in translational proteomics.Expert review of proteomics · 2016Review
- Personalized Proteomics: The Future of Precision Medicine.Proteomes · 2016Article
- Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification.Journal of visualized experiments : JoVE · 2015Article
- Practical immunoaffinity-enrichment LC-MS for measuring protein kinetics of low-abundance proteins.Clinical chemistry · 2014Article
- Rapid development of sensitive, high-throughput, quantitative and highly selective mass spectrometric targeted immunoassays for clinically important proteins in human plasma and serum.Clinical biochemistry · 2013Article
- Clinical review: improving the measurement of serum thyroglobulin with mass spectrometry.The Journal of clinical endocrinology and metabolism · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
For decades, immunoassays have provided the framework for protein biomarker studies in clinical medicine and in therapeutic monitoring for drug development. At the same time, investigators have uncovered many issues that make immunoassays unreliable in many human serum and plasma samples. LC-MS/MS after tryptic digestion of proteins is potentially an attractive solution, but the sensitivity of the method is not sufficient to measure many important low-abundance proteins directly. The use of antipeptide antibodies to immunoenrich peptides of interest can improve the sensitivity of the approach, greatly simplify the matrix enabling shortened chromatographic runs, and facilitate the multiplexed quantification of analytes, which could reduce the costs of quantitative protein measurements in complex specimens. We provide an overview of the method and the steps needed to develop an assay. In addition, we review the efforts to make this method generally more applicable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.