Evidence map›Paper›PMID 22303488›Full record

SynthesisPLoS neglected tropical diseases2012

Comparative study of rK39 Leishmania antigen for serodiagnosis of visceral leishmaniasis: systematic review with meta-analysis.

Zuinara Maia, Monique Lírio, Sóstenes Mistro, Carlos Maurício Cardeal Mendes, Sanjay R Mehta, Roberto Badaro

Open access · goldAbstract readComparative StudyEvaluation StudyMeta-Analysis
PubMed Full text at PMC Publisher
In one paragraph

Synthesis in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed, 6 pooled it
10.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed, 6 syntheses or guidelines pooled it, 137 citations in OpenAlex.

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  19. Evidence map of diagnosis, treatment, prognosis, prevention, and control in visceral leishmaniasis.Revista panamericana de salud publica = Pan American journal of public health · 2022
    Review
  20. Article

21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Zuinara MaiaDepartment of Medicine, Federal University of Bahia Salvador, Bahia, Brazil.
Monique Lírio
Sóstenes Mistro
Carlos Maurício Cardeal Mendes
Sanjay R Mehta
Roberto Badaro
Universidade Federal da Bahia · BRUniversity of California, San Diego · US

Funding

Molecular Epidemiology of HIV-1 in the California-Mexico Border RegionK23AI093163 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MEHTA, SANJAY R · 2011 to 2015
$643k
NIAID NIH HHS K23 AI093163
6 · The paper itself

Abstract

backgroundThe rK39 recombinant protein is derived from a specific antigen produced by the Leishmania donovani complex, and has been used in the last two decades for the serodiagnosis of visceral leishmaniasis. We present here a systematic review and meta-analysis of studies evaluating serologic assays to diagnose visceral leishmaniasis to determine the accuracy of rK39 antigen in comparison to the use of other antigen preparations. METHODOLOGY/PRINCIPAL

findingsA systematic review with meta-analysis of the literature was performed to compare the rK39 strip-test and ELISA formats against serological tests using promastigote antigens derived from whole or soluble parasites for Direct Aglutination Test (DAT), Indirect Immunofluorescence test (IFAT) and ELISA with a promastigote antigen preparation (p-ELISA). Gold standard diagnosis was defined by the demonstration of amastigotes on hematological specimens. A database search was performed on Medline, Lilacs, Scopus, Isi Web of Science, and Cochrane Library. Quality of data was assessed using the QUADAS questionnaire. A search of the electronic databases found 352 papers of which only 14 fulfilled the selection criteria. Three evaluated the rK39 ELISA, while 13 evaluated the rK39 immunochromatographic strip test. The summarized sensitivity for the rK39-ELISA was 92% followed by IFAT 88% and p-ELISA 87%. The summarized specificity for the three diagnostic tests was 81%, 90%, and 77%. Studies comparing the rK39 strip test with DAT found a similar sensitivity of 94%, although the DAT had a slightly higher specificity. The rK39 strip test was more sensitive and specific than the IFAT and p-ELISA. We did not detect any difference in the sensitivity and specificity between strips produced by different manufacturers.

conclusionsThe rK39 protein used either in a strip test or in an ELISA, and the DAT are the best choices for implementation of rapid, easy and efficient test for serodiagnosis of VL.

Indexed as

Antigens, ProtozoanProtozoan ProteinsHumansLeishmaniasis, VisceralParasitologyRecombinant ProteinsSensitivity and SpecificitySerologic TestsAntigens, ProtozoanK39 antigen, LeishmaniaProtozoan ProteinsRecombinant Proteins

Identifiers

PMID22303488
PMCPMC3269412
OpenAlexW2045730389

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.